E-ISSN 0976-2779 | ISSN 0975-8453
 

Review Article 


The Autophagy Inhibitor Hydroxychloroquine Enhances Sensitivity of Osteosarcoma Cell Line MG-63 to Doxorubicin Treatment

Hasanain K. Hasan Owadh, Bassim I. Mohammad, Sadiq J. Al-Muhana, Hamid Naji Obied, Rana A. Ghaleb, Najah R Hadi.

Abstract
Autophagy is a self-energy supplier process response to stressful or fasting conditions where cell degrades and recycles intracellular constituents as an alternative way of energy source. Unfortunately Autophagy process play important role in tumor cell resistance to anticancer drugs. Osteosarcoma is the most commonly diagnosed primary malignant tumor of the bone. The present study evaluates the role of Autophagy inhibitor hydroxychloroquine (HCQ) on doxorubicin-induced cell death in Osteosarcoma cell line (MG-63). The results of current study found that doxorubicin induced Autophagy in Osteosarcoma (MG-63) cells, exhibiting an increased microtubule associated protein 1 light chain 3 (LC3)level, a decrease in SQSTM1 (p62) level, moreover using of Autophagy modulator increased in generation of reactive oxygen species (ROS) and decreased Autophagy activity and increased Osteosarcoma-cell death.
Although doxorubicin-induced cell death was enhanced by combined with HCQ but at high concentration of doxorubicin the cytotoxicity was decreased with increased Autophagy activity. These finding suggest that Autophagy attenuate cytotoxicity effect of doxorubicin by decreasing level of ROS, while HCQ improved doxorubicin-cytotoxicity on Osteosarcoma by inhibiting of Autophagy process.

Key words: Osteosarcoma, Autophagy, doxorubicin, hydroxychloroquine, MG-63.


 
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How to Cite this Article
Pubmed Style

Hasanain K. Hasan Owadh, Bassim I. Mohammad, Sadiq J. Al-Muhana, Hamid Naji Obied, Rana A. Ghaleb, Najah R Hadi. The Autophagy Inhibitor Hydroxychloroquine Enhances Sensitivity of Osteosarcoma Cell Line MG-63 to Doxorubicin Treatment. SRP. 2020; 11(5): 63-69. doi:10.31838/srp.2020.5.11


Web Style

Hasanain K. Hasan Owadh, Bassim I. Mohammad, Sadiq J. Al-Muhana, Hamid Naji Obied, Rana A. Ghaleb, Najah R Hadi. The Autophagy Inhibitor Hydroxychloroquine Enhances Sensitivity of Osteosarcoma Cell Line MG-63 to Doxorubicin Treatment. http://www.sysrevpharm.org//?mno=106836 [Access: May 31, 2020]. doi:10.31838/srp.2020.5.11


AMA (American Medical Association) Style

Hasanain K. Hasan Owadh, Bassim I. Mohammad, Sadiq J. Al-Muhana, Hamid Naji Obied, Rana A. Ghaleb, Najah R Hadi. The Autophagy Inhibitor Hydroxychloroquine Enhances Sensitivity of Osteosarcoma Cell Line MG-63 to Doxorubicin Treatment. SRP. 2020; 11(5): 63-69. doi:10.31838/srp.2020.5.11



Vancouver/ICMJE Style

Hasanain K. Hasan Owadh, Bassim I. Mohammad, Sadiq J. Al-Muhana, Hamid Naji Obied, Rana A. Ghaleb, Najah R Hadi. The Autophagy Inhibitor Hydroxychloroquine Enhances Sensitivity of Osteosarcoma Cell Line MG-63 to Doxorubicin Treatment. SRP. (2020), [cited May 31, 2020]; 11(5): 63-69. doi:10.31838/srp.2020.5.11



Harvard Style

Hasanain K. Hasan Owadh, Bassim I. Mohammad, Sadiq J. Al-Muhana, Hamid Naji Obied, Rana A. Ghaleb, Najah R Hadi (2020) The Autophagy Inhibitor Hydroxychloroquine Enhances Sensitivity of Osteosarcoma Cell Line MG-63 to Doxorubicin Treatment. SRP, 11 (5), 63-69. doi:10.31838/srp.2020.5.11



Turabian Style

Hasanain K. Hasan Owadh, Bassim I. Mohammad, Sadiq J. Al-Muhana, Hamid Naji Obied, Rana A. Ghaleb, Najah R Hadi. 2020. The Autophagy Inhibitor Hydroxychloroquine Enhances Sensitivity of Osteosarcoma Cell Line MG-63 to Doxorubicin Treatment. Systematic Reviews in Pharmacy, 11 (5), 63-69. doi:10.31838/srp.2020.5.11



Chicago Style

Hasanain K. Hasan Owadh, Bassim I. Mohammad, Sadiq J. Al-Muhana, Hamid Naji Obied, Rana A. Ghaleb, Najah R Hadi. "The Autophagy Inhibitor Hydroxychloroquine Enhances Sensitivity of Osteosarcoma Cell Line MG-63 to Doxorubicin Treatment." Systematic Reviews in Pharmacy 11 (2020), 63-69. doi:10.31838/srp.2020.5.11



MLA (The Modern Language Association) Style

Hasanain K. Hasan Owadh, Bassim I. Mohammad, Sadiq J. Al-Muhana, Hamid Naji Obied, Rana A. Ghaleb, Najah R Hadi. "The Autophagy Inhibitor Hydroxychloroquine Enhances Sensitivity of Osteosarcoma Cell Line MG-63 to Doxorubicin Treatment." Systematic Reviews in Pharmacy 11.5 (2020), 63-69. Print. doi:10.31838/srp.2020.5.11



APA (American Psychological Association) Style

Hasanain K. Hasan Owadh, Bassim I. Mohammad, Sadiq J. Al-Muhana, Hamid Naji Obied, Rana A. Ghaleb, Najah R Hadi (2020) The Autophagy Inhibitor Hydroxychloroquine Enhances Sensitivity of Osteosarcoma Cell Line MG-63 to Doxorubicin Treatment. Systematic Reviews in Pharmacy, 11 (5), 63-69. doi:10.31838/srp.2020.5.11