E-ISSN 0976-2779 | ISSN 0975-8453
 

Review Article 


An Experimental Trial to Prepared γ1 34.5 Herpes Simplex Virus 1 Immunogene by cloning Technique

Ameer M Hadi, Shakir H. Mohammed Al-Alwany, Zaytoon A. Al-Khafaji.

Abstract
The herpes simplex virus (HSV) family offers particular advantages for use as a viral oncolytic. The engineered vectors that make up oncolytic HSVs (oHSVs) have demonstrated remarkable safety in clinical trials, with some evidence of efficacy. The past decade has seen a focus on increasing the efficacy of oncolytic vectors by adding exogenous transgenes to enhance tumor destruction.
This study aimed to prepared the Recombinant γ1 34.5 - Infected Cell Protein (ICP34.5) Herpes Simplex Virus-1 Immunogenic by cloning technique from the wild type hsv-1, through the following steps; firstly propagation the virus on Vero cell line o estimate the cytopathic effect; after then it was diagnosed by immunofluorescent microscope in cell culture supernatant; followed by evaluation the titration of hsv-1 by plaque assay. Secondly, used specific set of primers for (γ1 34.5 gene) sequence, then cleaved this sequence by restriction enzyme; followed it cloned this sequence by appropriate vector (PSL1180), then was mixed with another vector contain green fluorescent protein to ensure was gained these sequence. Thirdly, transport this sequence to vero cell line by specific transporter for development and produce there gene expression (ICP34.5) immunogenic.

Key words: HSV-1, cytopathic effect, γ1ICP34.5, vero cell line, Immunofluorescence


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Ameer M Hadi
Articles by Shakir H. Mohammed Al-Alwany
Articles by Zaytoon A. Al-Khafaji
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Ameer M Hadi, Shakir H. Mohammed Al-Alwany, Zaytoon A. Al-Khafaji. An Experimental Trial to Prepared γ1 34.5 Herpes Simplex Virus 1 Immunogene by cloning Technique. SRP. 2020; 11(5): 140-149. doi:10.31838/srp.2020.5.22


Web Style

Ameer M Hadi, Shakir H. Mohammed Al-Alwany, Zaytoon A. Al-Khafaji. An Experimental Trial to Prepared γ1 34.5 Herpes Simplex Virus 1 Immunogene by cloning Technique. http://www.sysrevpharm.org//?mno=108397 [Access: May 31, 2020]. doi:10.31838/srp.2020.5.22


AMA (American Medical Association) Style

Ameer M Hadi, Shakir H. Mohammed Al-Alwany, Zaytoon A. Al-Khafaji. An Experimental Trial to Prepared γ1 34.5 Herpes Simplex Virus 1 Immunogene by cloning Technique. SRP. 2020; 11(5): 140-149. doi:10.31838/srp.2020.5.22



Vancouver/ICMJE Style

Ameer M Hadi, Shakir H. Mohammed Al-Alwany, Zaytoon A. Al-Khafaji. An Experimental Trial to Prepared γ1 34.5 Herpes Simplex Virus 1 Immunogene by cloning Technique. SRP. (2020), [cited May 31, 2020]; 11(5): 140-149. doi:10.31838/srp.2020.5.22



Harvard Style

Ameer M Hadi, Shakir H. Mohammed Al-Alwany, Zaytoon A. Al-Khafaji (2020) An Experimental Trial to Prepared γ1 34.5 Herpes Simplex Virus 1 Immunogene by cloning Technique. SRP, 11 (5), 140-149. doi:10.31838/srp.2020.5.22



Turabian Style

Ameer M Hadi, Shakir H. Mohammed Al-Alwany, Zaytoon A. Al-Khafaji. 2020. An Experimental Trial to Prepared γ1 34.5 Herpes Simplex Virus 1 Immunogene by cloning Technique. Systematic Reviews in Pharmacy, 11 (5), 140-149. doi:10.31838/srp.2020.5.22



Chicago Style

Ameer M Hadi, Shakir H. Mohammed Al-Alwany, Zaytoon A. Al-Khafaji. "An Experimental Trial to Prepared γ1 34.5 Herpes Simplex Virus 1 Immunogene by cloning Technique." Systematic Reviews in Pharmacy 11 (2020), 140-149. doi:10.31838/srp.2020.5.22



MLA (The Modern Language Association) Style

Ameer M Hadi, Shakir H. Mohammed Al-Alwany, Zaytoon A. Al-Khafaji. "An Experimental Trial to Prepared γ1 34.5 Herpes Simplex Virus 1 Immunogene by cloning Technique." Systematic Reviews in Pharmacy 11.5 (2020), 140-149. Print. doi:10.31838/srp.2020.5.22



APA (American Psychological Association) Style

Ameer M Hadi, Shakir H. Mohammed Al-Alwany, Zaytoon A. Al-Khafaji (2020) An Experimental Trial to Prepared γ1 34.5 Herpes Simplex Virus 1 Immunogene by cloning Technique. Systematic Reviews in Pharmacy, 11 (5), 140-149. doi:10.31838/srp.2020.5.22