E-ISSN 0976-2779 | ISSN 0975-8453
 

Review Article 


Purification of Urokinase Enzyme from Serum Blood of Patients with Pneumonia

Aya Jasim Mohammed, Asmaa Hashim Shaker.

Abstract
Among the most common lung diseases such as Tuberculosis, Chronic Obstructive Pulmonary, Asthma, Lung Cancer, Cystic fibrosis, etc., Pneumonia includes all lung diseases that affect the airway and other parts of the lung. Urokinase (UK) [EC 3.4.21.73] is strong plasminogen activator, also known urokinase type plasminogen activator (uPA), which is serine protein. that activates an inactive proenzyme plasminogen to an active plasmin via cleavage of Arg–Val bond. The active plasmin causes break down the fibrin polymers of blood clots. In this study, the UK enzyme was purified from serum blood of patients with pneumonia and the activity of the purified enzyme were measured measured. The purification step includes the precipitation by Ammonium sulfate salt saturation (60%) followed by isolating the enzyme using chromatography Gel filtration by sephadex G–100 and DEAE–Cellulose ion exchange to produce iso-enzyme. The apparent molecular weight of the isolated UK using SDS–PAGE. The results show that the enzyme activity values in male patients is 191.0 ± 11.8 (I.U.) while in female patients is 158.1± 9.59 (I.U.). The enzyme activity in male control 87.8 ± 5.9 (I.U.) and Female 82.5 ± 4.15 (I.U.). Further, the kinetics of the purified enzyme suggest that the value of the reaction rate (Km) is 0.4 M. The enzymes shown an optimum pH at 8.8, and optimum temperature at 37 Cº.

Key words: Pneumonia, Urokinase enzyme, Purification


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Aya Jasim Mohammed
Articles by Asmaa Hashim Shaker
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Aya Jasim Mohammed, Asmaa Hashim Shaker. Purification of Urokinase Enzyme from Serum Blood of Patients with Pneumonia. SRP. 2020; 11(6): 824-830. doi:10.31838/srp.2020.6.119


Web Style

Aya Jasim Mohammed, Asmaa Hashim Shaker. Purification of Urokinase Enzyme from Serum Blood of Patients with Pneumonia. http://www.sysrevpharm.org//?mno=119072 [Access: June 26, 2020]. doi:10.31838/srp.2020.6.119


AMA (American Medical Association) Style

Aya Jasim Mohammed, Asmaa Hashim Shaker. Purification of Urokinase Enzyme from Serum Blood of Patients with Pneumonia. SRP. 2020; 11(6): 824-830. doi:10.31838/srp.2020.6.119



Vancouver/ICMJE Style

Aya Jasim Mohammed, Asmaa Hashim Shaker. Purification of Urokinase Enzyme from Serum Blood of Patients with Pneumonia. SRP. (2020), [cited June 26, 2020]; 11(6): 824-830. doi:10.31838/srp.2020.6.119



Harvard Style

Aya Jasim Mohammed, Asmaa Hashim Shaker (2020) Purification of Urokinase Enzyme from Serum Blood of Patients with Pneumonia. SRP, 11 (6), 824-830. doi:10.31838/srp.2020.6.119



Turabian Style

Aya Jasim Mohammed, Asmaa Hashim Shaker. 2020. Purification of Urokinase Enzyme from Serum Blood of Patients with Pneumonia. Systematic Reviews in Pharmacy, 11 (6), 824-830. doi:10.31838/srp.2020.6.119



Chicago Style

Aya Jasim Mohammed, Asmaa Hashim Shaker. "Purification of Urokinase Enzyme from Serum Blood of Patients with Pneumonia." Systematic Reviews in Pharmacy 11 (2020), 824-830. doi:10.31838/srp.2020.6.119



MLA (The Modern Language Association) Style

Aya Jasim Mohammed, Asmaa Hashim Shaker. "Purification of Urokinase Enzyme from Serum Blood of Patients with Pneumonia." Systematic Reviews in Pharmacy 11.6 (2020), 824-830. Print. doi:10.31838/srp.2020.6.119



APA (American Psychological Association) Style

Aya Jasim Mohammed, Asmaa Hashim Shaker (2020) Purification of Urokinase Enzyme from Serum Blood of Patients with Pneumonia. Systematic Reviews in Pharmacy, 11 (6), 824-830. doi:10.31838/srp.2020.6.119





Most Viewed Articles
  • Dental Development between Assisted Reproductive Therapy (Art) and Natural Conceived Children: A Comparative Pilot Study
    Norzaiti Mohd Kenali, Naimah Hasanah Mohd Fathil, Norbasyirah Bohari, Ahmad Faisal Ismail, Roszaman Ramli
    SRP. 2020; 11(1): 01-06
    » Abstract » doi: 10.5530/srp.2020.1.01

  • Psychometric properties of the World Health Organization Quality of life instrument, short form: Validity in the Vietnamese healthcare context
    Trung Quang Vo*, Bao Tran Thuy Tran, Ngan Thuy Nguyen, Tram ThiHuyen Nguyen, Thuy Phan Chung Tran
    SRP. 2020; 11(1): 14-22
    » Abstract » doi: 10.5530/srp.2020.1.03

  • A Prospective Review on Phyto-Pharmacological Aspects of Andrographis paniculata
    Govindraj Akilandeswari, Arumugam Vijaya Anand, Palanisamy Sampathkumar, Puthamohan Vinayaga Moorthi, Basavaraju Preethi
    SRP. 2019; 10(1): 15-19
    » Abstract » doi: 10.5530/srp.2019.1.3

  • A Review of Pharmacoeconomics: the key to “Healthcare for All”
    Hasamnis AA, Patil SS, Shaik Imam, Narendiran K
    SRP. 2019; 10(1): s40-s42
    » Abstract » doi: 10.5530/srp.2019.1s.21

  • Deuterium Depleted Water as an Adjuvant in Treatment of Cancer
    Anton Syroeshkin, Olga Levitskaya, Elena Uspenskaya, Tatiana Pleteneva, Daria Romaykina, Daria Ermakova
    SRP. 2019; 10(1): 112-117
    » Abstract » doi: 10.5530/srp.2019.1.19

  • Most Downloaded
  • Dental Development between Assisted Reproductive Therapy (Art) and Natural Conceived Children: A Comparative Pilot Study
    Norzaiti Mohd Kenali, Naimah Hasanah Mohd Fathil, Norbasyirah Bohari, Ahmad Faisal Ismail, Roszaman Ramli
    SRP. 2020; 11(1): 01-06
    » Abstract » doi: 10.5530/srp.2020.1.01

  • Manilkara zapota (L.) Royen Fruit Peel: A Phytochemical and Pharmacological Review
    Karle Pravin P, Dhawale Shashikant C
    SRP. 2019; 10(1): 11-14
    » Abstract » doi: 0.5530/srp.2019.1.2

  • Pharmacognostic and Phytopharmacological Overview on Bombax ceiba
    Pankaj Haribhau Chaudhary, Mukund Ganeshrao Tawar
    SRP. 2019; 10(1): 20-25
    » Abstract » doi: 10.5530/srp.2019.1.4

  • A Review of Pharmacoeconomics: the key to “Healthcare for All”
    Hasamnis AA, Patil SS, Shaik Imam, Narendiran K
    SRP. 2019; 10(1): s40-s42
    » Abstract » doi: 10.5530/srp.2019.1s.21

  • A Prospective Review on Phyto-Pharmacological Aspects of Andrographis paniculata
    Govindraj Akilandeswari, Arumugam Vijaya Anand, Palanisamy Sampathkumar, Puthamohan Vinayaga Moorthi, Basavaraju Preethi
    SRP. 2019; 10(1): 15-19
    » Abstract » doi: 10.5530/srp.2019.1.3

  • Most Cited Articles