E-ISSN 0976-2779 | ISSN 0975-8453
 

Review Article 


Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry

Seher A. Almedeny, Khalida K. Abbas Al-Kelaby, Sarmad N. Gany, Hussein A. Abdul Hussein.

Abstract
The flow cytometry capability for fast and individualized analysis of a big number of cells would seem to make it typically selected for the cell death assessment. It offers the capability to revise large numbers cells separately rather than a mixed population, and investigates concurrently many proteins expression like cell specific markers and apoptotic indicators. Annexin V assay is one of the most widely used approach to detect apoptosis as it is easy-to-use, fast , needs no fixation and allows a large number of cells to be analyzed at any one time. Annexin assay is ideal for solitary cell suspensions and so flow cytometry. In this study we try to evaluate the apoptotic effect of the investigational drug TAN on human cancer cell line HCT116 in comparison with the triazoles: itraconazole (ITC), difenoconazole (DIF), epoxyconazole (EPO), and the standard anticancer agent’s doxorubicin (DOX) and 5-fluorouracil (5FU) by flow cytometry. To assess the apoptotic effect of the triazole analog TAN on human colorectal cancer cell line HCT116using annexin V dye via flow cytometry assay. The results of flow cytometry reveal a significant differences between treated and untreated HCT116 cells in all drugs based on mean ±Std. in all drugs that are used in this study , TAN showed a significant apoptotic effect after DOX ,DIF and EPO with P value = . 0005, the following conclusions as the TAN has significant and promising anticancerrole through induction of apoptosis in HCT116 cells and flow cytometry is a reliable technique to measure the apoptotic percentages in cells.

Key words: apopotic effect, Colorectal Cancer, Flow Cyclometry


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Seher A. Almedeny
Articles by Khalida K. Abbas Al-Kelaby
Articles by Sarmad N. Gany
Articles by Hussein A. Abdul Hussein
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein. Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry. SRP. 2019; 10(1): 214-219. doi:10.5530/srp.2019.1.37


Web Style

Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein. Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry. http://www.sysrevpharm.org//?mno=302644975 [Access: June 01, 2020]. doi:10.5530/srp.2019.1.37


AMA (American Medical Association) Style

Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein. Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry. SRP. 2019; 10(1): 214-219. doi:10.5530/srp.2019.1.37



Vancouver/ICMJE Style

Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein. Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry. SRP. (2019), [cited June 01, 2020]; 10(1): 214-219. doi:10.5530/srp.2019.1.37



Harvard Style

Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein (2019) Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry. SRP, 10 (1), 214-219. doi:10.5530/srp.2019.1.37



Turabian Style

Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein. 2019. Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry. Systematic Reviews in Pharmacy, 10 (1), 214-219. doi:10.5530/srp.2019.1.37



Chicago Style

Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein. "Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry." Systematic Reviews in Pharmacy 10 (2019), 214-219. doi:10.5530/srp.2019.1.37



MLA (The Modern Language Association) Style

Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein. "Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry." Systematic Reviews in Pharmacy 10.1 (2019), 214-219. Print. doi:10.5530/srp.2019.1.37



APA (American Psychological Association) Style

Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein (2019) Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry. Systematic Reviews in Pharmacy, 10 (1), 214-219. doi:10.5530/srp.2019.1.37





Most Viewed Articles
  • Dental Development between Assisted Reproductive Therapy (Art) and Natural Conceived Children: A Comparative Pilot Study
    Norzaiti Mohd Kenali, Naimah Hasanah Mohd Fathil, Norbasyirah Bohari, Ahmad Faisal Ismail, Roszaman Ramli
    SRP. 2020; 11(1): 01-06
    » Abstract » doi: 10.5530/srp.2020.1.01

  • Psychometric properties of the World Health Organization Quality of life instrument, short form: Validity in the Vietnamese healthcare context
    Trung Quang Vo*, Bao Tran Thuy Tran, Ngan Thuy Nguyen, Tram ThiHuyen Nguyen, Thuy Phan Chung Tran
    SRP. 2020; 11(1): 14-22
    » Abstract » doi: 10.5530/srp.2020.1.03

  • A Prospective Review on Phyto-Pharmacological Aspects of Andrographis paniculata
    Govindraj Akilandeswari, Arumugam Vijaya Anand, Palanisamy Sampathkumar, Puthamohan Vinayaga Moorthi, Basavaraju Preethi
    SRP. 2019; 10(1): 15-19
    » Abstract » doi: 10.5530/srp.2019.1.3

  • A Review of Pharmacoeconomics: the key to “Healthcare for All”
    Hasamnis AA, Patil SS, Shaik Imam, Narendiran K
    SRP. 2019; 10(1): s40-s42
    » Abstract » doi: 10.5530/srp.2019.1s.21

  • Deuterium Depleted Water as an Adjuvant in Treatment of Cancer
    Anton Syroeshkin, Olga Levitskaya, Elena Uspenskaya, Tatiana Pleteneva, Daria Romaykina, Daria Ermakova
    SRP. 2019; 10(1): 112-117
    » Abstract » doi: 10.5530/srp.2019.1.19

  • Most Downloaded
  • Dental Development between Assisted Reproductive Therapy (Art) and Natural Conceived Children: A Comparative Pilot Study
    Norzaiti Mohd Kenali, Naimah Hasanah Mohd Fathil, Norbasyirah Bohari, Ahmad Faisal Ismail, Roszaman Ramli
    SRP. 2020; 11(1): 01-06
    » Abstract » doi: 10.5530/srp.2020.1.01

  • Manilkara zapota (L.) Royen Fruit Peel: A Phytochemical and Pharmacological Review
    Karle Pravin P, Dhawale Shashikant C
    SRP. 2019; 10(1): 11-14
    » Abstract » doi: 0.5530/srp.2019.1.2

  • Pharmacognostic and Phytopharmacological Overview on Bombax ceiba
    Pankaj Haribhau Chaudhary, Mukund Ganeshrao Tawar
    SRP. 2019; 10(1): 20-25
    » Abstract » doi: 10.5530/srp.2019.1.4

  • A Review of Pharmacoeconomics: the key to “Healthcare for All”
    Hasamnis AA, Patil SS, Shaik Imam, Narendiran K
    SRP. 2019; 10(1): s40-s42
    » Abstract » doi: 10.5530/srp.2019.1s.21

  • A Prospective Review on Phyto-Pharmacological Aspects of Andrographis paniculata
    Govindraj Akilandeswari, Arumugam Vijaya Anand, Palanisamy Sampathkumar, Puthamohan Vinayaga Moorthi, Basavaraju Preethi
    SRP. 2019; 10(1): 15-19
    » Abstract » doi: 10.5530/srp.2019.1.3

  • Most Cited Articles