Abstract
Phaleria macrocarpa, is widely known as Mahkota dewa is a herb which is used in medical treatments and found in Malaysia & Indonesia. Extract of P. Macrocarpa contains a chemical composition which is useful in anti-cancerous, anti-fungus, anti-oxidation, anti-diabetic treatments. Reactive oxygen species (ROS) production in mammalian cell is responsible for many cell function, absence of detoxification for removal of this radical, affects cell functions and may produce abnormalities and diseases. Several nervous abnormalities were found be to the effect of ROS generation. Currently there are no effective medicinal options for its treatment. This study focuses on the effect of P. Macrocarpa leaf extract on the restraining reactive oxygen.
Methods: P. Macrocarpa leaf extract contains 80% methyl alcohol. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) is used for cytotoxicity analysis and restraining reactive oxygen is studied using 2′,7′-Dichlorofluorescin diacetate in SK-N-SH cell model.
Result: ROS Restraining performance of leaf extract is analysed between the group that treated only with crude extract, group treated with extract with Hydrogen peroxide and group treated only with Hydrogen peroxide. HPLC methods shows that Vitexin & iso-vitexin, this bioactive compound were present in the crude extract at retention time 20.98 & 22.94 for Vitexin & iso-vitexin respectively.
Conclusion: Anti-oxidation & low toxic characteristic of P. Macrocarpa extract concludes it to be beneficial for its usage as herbal supplement/medicine.

Key words: Restraining effect of P. macrocarpa, methanol extract on ROS, generation in SK-N-SH cell