Abstract

Amel H. Mussa, Afrah Fahad Abdulkareem, Hanaa Farhan Abbas.

Pseudomonas putida considered one of the most important microbes were used in biological control against a number of soil borne diseases because of its ability to produce large number of antimicrobial agents against pathogenic bacteria and Fungi.
This study designed to isolate the P. putida from Iraqi soil of different plant roots rhizosphere, and identified the isolates by culturing as well as by morphological and biochemical testing , vitek 2 system using to confirmed the acquaintance of isolates, then extracted and purified active metabolites phenazine -1- carboxylic acid were produced from P. putida and measured the activity in vitro; via inhibition the growth of some pathogenic MDR bacteria causing urinary tract infection .
The effectiveness of phenazine -1- carboxylic acid was studied on DNA (deoxy ribonucleic acid) extracted from some pathogenic bacteria ; as well as the standard calf thymus gland double stranded ctds DNA.
The results obtained in this work showed That active compound phenazine -1- carboxylic acid had effect on the DNA extracted from calf thymus gland, caused (Hyperchromasia), referring to cleavage the double standard DNA and causing the (Hypochromasia) breakage DNA extracted from Acinetobacter baumannii, Staphylococcus aureus. This study concluded that the P.putida has inhibitory effect on the growth of pathogenic bacteria and the mode of action of its activity could be relevant due to the production of antibiotics such as phenazine -1- carboxylic acid and subpart to its direct effect on DNA.