Abstract

Elena V. Maslova, Tatyana V. Glodik, Elena & 1040; Kalashnikova, Irina V. Spichak, Tatyana & 1040 ;. Perelygina

The antibacterial activity of Ballota nigra L. callus tissue obtained in vitro against Escherichia coli and Staphylococcus aureus was studied. The sterilization mode of plant explants was optimized when introduced into an in vitro culture. The most effective nutrient media for the growth of B. nigra cell cultures were selected. A comparative analysis of the antibacterial activity of plant extracts obtained from callus tissues and intact plants was carried out. It was found that plant extracts from callus tissue B.nigra and all its dilutions had greater antimicrobial activity against E. coli than extracts from parts of an intact plant (flowers and leaves) and 100% extracts from callus tissues and their dilution 1:10 and 1: 100 exhibited a practically similar antibacterial effect to S. aureus, as did an intact plant.