Colorimetric Determination Of Sitagliptin As An Oxidation Derivative Of Ninhydrin
Abstract
Ali Khalil Mahmood, Taghreed N-A Omar, Khalid Waleed S. Al-Janabi.
A sensitive and accurate colorimetric method was developed for the determination of the Sitagliptin phosphate monohydrate, here and after will be named Sitagliptin, in its pure and pharmaceutical form. The suggested approach is based on boosting the sensitivity of the traditional spectrometric methods by derivatizing Sitagliptin into a colored product that absorbs the visible spectrum at 573 nm.
The proposed method has effectively improved the sensitivity and the limit of detection for the analysis of Sitagliptin.
A linear calibration curve was obtained over the concentration range of 0.1-10 μg/ml with a correlation coefficient of 0.9983. The calculated recovery was within the range of 98.98 – 100.11%. While the limit of detection LOD and the limit of quantification LOQ were 0.03 mg/L and 0.1 mg/L respectively.
The established procedure has been validated as exhibited linear relationship of spectroscopic absorbance intensity versus analyte concentrations and can be applied for routine quantification of the active constituent of Sitagliptin in different pharmaceuticals.
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| Pubmed Style Ali Khalil Mahmood, Taghreed N-A Omar, Khalid Waleed S. Al-Janabi. Colorimetric Determination Of Sitagliptin As An Oxidation Derivative Of Ninhydrin. SRP. 2021; 12(2): 175-178. doi:10.31838/srp.2021.2.22 Web Style Ali Khalil Mahmood, Taghreed N-A Omar, Khalid Waleed S. Al-Janabi. Colorimetric Determination Of Sitagliptin As An Oxidation Derivative Of Ninhydrin. http://www.sysrevpharm.org/?mno=13740 [Access: March 29, 2021]. doi:10.31838/srp.2021.2.22 AMA (American Medical Association) Style Ali Khalil Mahmood, Taghreed N-A Omar, Khalid Waleed S. Al-Janabi. Colorimetric Determination Of Sitagliptin As An Oxidation Derivative Of Ninhydrin. SRP. 2021; 12(2): 175-178. doi:10.31838/srp.2021.2.22 Vancouver/ICMJE Style Ali Khalil Mahmood, Taghreed N-A Omar, Khalid Waleed S. Al-Janabi. Colorimetric Determination Of Sitagliptin As An Oxidation Derivative Of Ninhydrin. SRP. (2021), [cited March 29, 2021]; 12(2): 175-178. doi:10.31838/srp.2021.2.22 Harvard Style Ali Khalil Mahmood, Taghreed N-A Omar, Khalid Waleed S. Al-Janabi (2021) Colorimetric Determination Of Sitagliptin As An Oxidation Derivative Of Ninhydrin. SRP, 12 (2), 175-178. doi:10.31838/srp.2021.2.22 Turabian Style Ali Khalil Mahmood, Taghreed N-A Omar, Khalid Waleed S. Al-Janabi. 2021. Colorimetric Determination Of Sitagliptin As An Oxidation Derivative Of Ninhydrin. Systematic Reviews in Pharmacy, 12 (2), 175-178. doi:10.31838/srp.2021.2.22 Chicago Style Ali Khalil Mahmood, Taghreed N-A Omar, Khalid Waleed S. Al-Janabi. "Colorimetric Determination Of Sitagliptin As An Oxidation Derivative Of Ninhydrin." Systematic Reviews in Pharmacy 12 (2021), 175-178. doi:10.31838/srp.2021.2.22 MLA (The Modern Language Association) Style Ali Khalil Mahmood, Taghreed N-A Omar, Khalid Waleed S. Al-Janabi. "Colorimetric Determination Of Sitagliptin As An Oxidation Derivative Of Ninhydrin." Systematic Reviews in Pharmacy 12.2 (2021), 175-178. Print. doi:10.31838/srp.2021.2.22 APA (American Psychological Association) Style Ali Khalil Mahmood, Taghreed N-A Omar, Khalid Waleed S. Al-Janabi (2021) Colorimetric Determination Of Sitagliptin As An Oxidation Derivative Of Ninhydrin. Systematic Reviews in Pharmacy, 12 (2), 175-178. doi:10.31838/srp.2021.2.22 |
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