Comparative Restriction Analysis of the Genomes of the Blastocystis spp Strains.

Abstract

Bugero Nina Vladimirovna, Ilyina Natalia Anatolievna, Alexandrova Svetlana Mikhailovna

The discussion about the clinical significance of Blastocystis spp. and its pathogenic potential is still ongoing. In recent decades, high rates of blastocyst insemination have been identified in various population groups. It is known that, being in the intestine, blastocysts participate in the formation of the microbiome of the ecotope. Disrupting the balance of microorganisms, these pathogens contribute to the creation of favorable conditions for the development of pathological processes. However, the mechanisms that reduce the protective forces of the macroorganism and contribute to the development and flow of the epidemiological process require deeper knowledge in addressing this issue. In the presented work a relatively simple way of determining a blastocyst with different degree of virulence using modern restriction analysis of the protozoa genome has been proposed. In the genome of blastocyst, a genetically determined number of recognition sites for a certain type of restriction enzymes was found, which led to the formation of a strictly defined number of DNA fragments of a fixed size in the study groups of protozoans. The proposed combination of endonucleases (Hae III, Pst I and Hind III) allowed to carry out species identification of strains of Blastocystis spp. differing in virulence indices, and also to judge about presence of different phenotypic groups of the protozoans in the studied biotope.