Comparison of Jatropha curcas (Linn) Leaf Extract and Nystatin Effectivity on Candida albicans Growth Inhibition

Abstract

Ali Yusran, Fuad Husain Akbar, Fathimah.

Fungal infection is a health problem that is faced around the world and arose in developing countries. Oral candidiasis is a fungal infection that affects oral mucosa. Lesion of oral candidiasis is caused by Candida albicans, which is considered as an opportunistic infection in humans. Antifungal therapy is necessary to inhibit the growth of these fungi. Traditional medicine becomes the alternative therapy to face this. One of Traditional therapy is Jatropha curcas (Linn) leaf. Jatropha curcas (Linn) leaf contains natural substances like flavonoid, tannin, and saponin that function as Antifungal. To measure the effectivity comparison between 50% concentration of Jatropha curcas (Linn) leaf extract with 100.000 IU of nystatin to the growth of Candida C. albicans. The research method is a laboratory experiment. The design of this study was posttest only control group design with agar / Kirby Bauer diffusion method. In this research, nine repetitions were carried out on three inhibitory treatment groups using 50% concentration of Jatropha curcas (Linn) leaf extract, 100.000 IU nystatin, and control (sterile aquades). The instrument in this research is a caliper with a millimeters (mm) unit. Shapiro wilk normality test showed p>0.05 to all groups. It shows that all groups were normally distributed. So, it fulfills the parametric test requirement, namely one-way ANOVA. This One-Way ANOVA parametric test indicated whether or not there is differentiation among the individuals that given different treatments. From the test, we can conclude that there is a different inhibitory test between 50% concentration of Jatropha curcas (Linn) leaf extract with nystatin to the growth of C. albicans. Nystatin is more effective rather than Jatropha curcas (Linn) leaf extract. But Jatropha curcas (Linn) leaf extract has the potential to inhibit the growth of C. albicans so that it can be taken into consideration as an alternative drug for the growth of the Candida C. albicans.