Abstract

Saif Jabbar Yasir, Younis Abdul-Redha AL- Khafaji, Khalida K Abbas Al-Kelaby

 

The case-control diagnostic study was developed to evaluate the prevalence of Human cytomegalovirus (HCMV) among the population of pregnant women (160 women of ages ranging from 15 -45 years old who were attended to get medical diagnosis at Al-Najaf public health laboratory, during a period extending from 15th January - 15th August 2019), and also 50 healthy pregnant women were included as a control group. Data about frequency of abortion, age, and residency were recorded. Diagnosis was firstly depending on Enzyme immunoassay (EIA) for the evaluation of the prevalence of anti- HCMV IgM and IgG antibodies in sera of pregnant women, then confirmed by the quantitative real-time PCR(qRT-PCR), using specific primers targeting of UL83 gene of HCMV. Results presented in this study showed that the overall prevalence of HCMV infection was 97:160 (60.63%) and among women of mean ages± Standard deviation (SD) equal to 26.243± 6.485. Out of 160 participant women; 95(59.38%) of cases had chronic HCMV infection while only 2 cases (1.25%) had acute HCMV infection. Results were revealing that 52:160 (32.5%) of HCMV infected cases were related to women of 16-25 years age group followed by those related to 26-35 years age group with a prevalence of 21.88%. Eight cases (5%) were related to 36-45 years age group, while only 2 cases (1.25%) had 15 years old age. Current results were also revealed that 35:59 (59.3%) from rural area, while 62:101 (61.4%) were from urban areas in Al-Najaf.Recurrent abortion (two or more frequency) was recorded to 17:97 (17.53%) of HCMV seropositivity cases with significant correlation. Results of HCMV UL83 gene amplification by qRT-PCR were showing that the overall prevalence of HCMV UL83 gene was 85% (136:160) of the total diagnostic group, and also positive in 25:50 (50%) of healthy pregnant women, with significant statistically differences. The HCMV positive threshold cycle (CT) of UL83 gene was revealing mean ± SD of 19.0537±6.64501, and 4 fold ratio as compared with that detected in asymptomatic cases by the method of 2^-∆∆CT. The interassay coefficient of variation (CV) of HCMV UL83 gene amplification CT was 34.88%. The qRT-PCR of HCMV UL83 amplification was revealing sensitivity, specificity and diagnostic accuracy of 100%, 96% and 99.4% respectively. While positive predictive and negative predictive values were equal to 99.3% and 100% respectively. We concluded that UL83 gene detection was successful, confirmatory to EIA and more précised for HCMV isolated from serum samples.

 

How to Cite this Article

Pubmed Style Saif Jabbar Yasir, Younis Abdul-Redha AL- Khafaji, Khalida K Abbas Al-Kelaby. Cytomegalovirus UL83 Gene as Diagnostic Marker for Preliminary Infection in Pregnant Women in AL-Najaf City. SRP. 2020; 11(5): 123-129. doi:10.31838/srp.2020.5.20 Web Style Saif Jabbar Yasir, Younis Abdul-Redha AL- Khafaji, Khalida K Abbas Al-Kelaby. Cytomegalovirus UL83 Gene as Diagnostic Marker for Preliminary Infection in Pregnant Women in AL-Najaf City. http://www.sysrevpharm.org/?mno=107668 [Access: March 29, 2021]. doi:10.31838/srp.2020.5.20 AMA (American Medical Association) Style Saif Jabbar Yasir, Younis Abdul-Redha AL- Khafaji, Khalida K Abbas Al-Kelaby. Cytomegalovirus UL83 Gene as Diagnostic Marker for Preliminary Infection in Pregnant Women in AL-Najaf City. SRP. 2020; 11(5): 123-129. doi:10.31838/srp.2020.5.20 Vancouver/ICMJE Style Saif Jabbar Yasir, Younis Abdul-Redha AL- Khafaji, Khalida K Abbas Al-Kelaby. Cytomegalovirus UL83 Gene as Diagnostic Marker for Preliminary Infection in Pregnant Women in AL-Najaf City. SRP. (2020), [cited March 29, 2021]; 11(5): 123-129. doi:10.31838/srp.2020.5.20 Harvard Style Saif Jabbar Yasir, Younis Abdul-Redha AL- Khafaji, Khalida K Abbas Al-Kelaby (2020) Cytomegalovirus UL83 Gene as Diagnostic Marker for Preliminary Infection in Pregnant Women in AL-Najaf City. SRP, 11 (5), 123-129. doi:10.31838/srp.2020.5.20 Turabian Style Saif Jabbar Yasir, Younis Abdul-Redha AL- Khafaji, Khalida K Abbas Al-Kelaby. 2020. Cytomegalovirus UL83 Gene as Diagnostic Marker for Preliminary Infection in Pregnant Women in AL-Najaf City. Systematic Reviews in Pharmacy, 11 (5), 123-129. doi:10.31838/srp.2020.5.20 Chicago Style Saif Jabbar Yasir, Younis Abdul-Redha AL- Khafaji, Khalida K Abbas Al-Kelaby. "Cytomegalovirus UL83 Gene as Diagnostic Marker for Preliminary Infection in Pregnant Women in AL-Najaf City." Systematic Reviews in Pharmacy 11 (2020), 123-129. doi:10.31838/srp.2020.5.20 MLA (The Modern Language Association) Style Saif Jabbar Yasir, Younis Abdul-Redha AL- Khafaji, Khalida K Abbas Al-Kelaby. "Cytomegalovirus UL83 Gene as Diagnostic Marker for Preliminary Infection in Pregnant Women in AL-Najaf City." Systematic Reviews in Pharmacy 11.5 (2020), 123-129. Print. doi:10.31838/srp.2020.5.20 APA (American Psychological Association) Style Saif Jabbar Yasir, Younis Abdul-Redha AL- Khafaji, Khalida K Abbas Al-Kelaby (2020) Cytomegalovirus UL83 Gene as Diagnostic Marker for Preliminary Infection in Pregnant Women in AL-Najaf City. Systematic Reviews in Pharmacy, 11 (5), 123-129. doi:10.31838/srp.2020.5.20