Abstract

Hasanain H. Ali, Sarmad N.Gany, Najah R. Hadi, Rihab H. Al-Mudhafar, Dhefaf H. Al-Mudhafar

Background: Hair loss is considered important dermatological condition happened in both sexes but more affected on male. Hair loss is complex situation correlated with cosmetics issues especially in women. The current regimes have numerous limitations and serous side effect, so should be searching more convenient therapy with less side effect and more efficacy. The recent studies illustrated the positive correlation of elevation the level of total antioxidant status with improvement hair regeneration in hair follicle. Also the current research revealed the significant correlation between reduction of lipid peroxidation markers like F2 isoprostane and enhancement of hair growth. Melatonin has indoleamine molecule with antioxidant and vasodilatation activities. It has a central role in improvement hair growth by acting as capturing of ROS and decreasing both DNA damage and apoptosis mechanism via antioxidant properties. Objective: This research aimed to investigate the hair regeneration effect of melatonin in reducing hair loss in mice model by serial photographs, histological examination and measuring of peroxidation and oxidative stress markers (F2 isoprostane, total antioxidant activity) by Elisa assay in animal model and assess the efficacy for them. Materials and Method: In this study, 20 adult Wister Albino mice were used with 6-7 weeks in age and weighing 25-35 g. Coat hairs on the dorsal skin of male mice were gently clipped by electrical machine and then stained by using commercial dye. The adult mice were divided randomly into equal 4 groups (5 mice in each group) as the following:
1. Ethanol group: Considered as a control group, All 5 mice treated with the vehicle solution absolute ethanol alcohol, 0.3 ml applied by a micropipette to the uncover skin then spread by means of swab once daily for 3 weeks. Then skin tissue was collected after 3 weeks of study.
2. Minoxidil treated group: All 5 mice treated with Minoxidil solution 5%, 0.3 ml was applied to the denuded skin by a micropipette once daily for 3 weeks for. Then skin tissue was collected after 3 weeks of study.
3. Melatonin treated group: All 5 mice treated with MT solution 0.1%, 0.3 ml was applied to the denuded skin by a micropipette once daily for 3 weeks. Then skin tissue was collected after 3 weeks of application.
4. Melatonin plus Minoxidil treated group: All 5 mice treated with equal amounts of MX solution 5% + MT 0.1%, 0.3 ml of the mixed solutions is applied by a micropipette once daily for 3 weeks for all 5 mice. Then skin tissue was collected after 3 weeks of application.
Results: At the end of this experimental study, we showed that the tissue level of F2-IsoPs in MX, MT and MT plus MX groups are significantly lowered than those in control (Ethanol) groups. Also we showed that the serum tissue level of TAC in MX, MT and MT plus MX groups is significantly higher than those of control (Ethanol) groups, but the MT and MT plus MX treated group are more significant than MX treated group in elevation of level TAC. On the other hand, we showed that the hair growth and enlargement of hair follicle in MX, MT and MT plus MX groups are significantly increased than those in control groups. Also we showed that the tissue level of is significantly higher than those of both control and vehicle groups. In histology examination, we revealed that the MT and MX are resulted insignificantly increase in number of hair follicle in comparison with control, however, the number of hair follicle significantly increased in group treated with combination of MT plus MX when compared to control group. Conclusion: From the overall results, we approved that the Melatonin is significantly reduce hair loss in adult male mice via their pleiotropic effects as anti-estrogenic, anti-oxidant and vasodilatation activities.

 

How to Cite this Article

Pubmed Style Hasanain H. Ali, Sarmad N.Gany, Najah R. Hadi, Rihab H. Al-Mudhafar, Dhefaf H. Al-Mudhafar. Effect Of Topically Applied Melatonin And Their Combination With Minoxidil Solution For Enhancement Of Hair Growth In Male Mice. SRP. 2021; 12(1): 402-410. doi:10.31838/srp.2021.1.61 Web Style Hasanain H. Ali, Sarmad N.Gany, Najah R. Hadi, Rihab H. Al-Mudhafar, Dhefaf H. Al-Mudhafar. Effect Of Topically Applied Melatonin And Their Combination With Minoxidil Solution For Enhancement Of Hair Growth In Male Mice. http://www.sysrevpharm.org/?mno=36832 [Access: March 30, 2021]. doi:10.31838/srp.2021.1.61 AMA (American Medical Association) Style Hasanain H. Ali, Sarmad N.Gany, Najah R. Hadi, Rihab H. Al-Mudhafar, Dhefaf H. Al-Mudhafar. Effect Of Topically Applied Melatonin And Their Combination With Minoxidil Solution For Enhancement Of Hair Growth In Male Mice. SRP. 2021; 12(1): 402-410. doi:10.31838/srp.2021.1.61 Vancouver/ICMJE Style Hasanain H. Ali, Sarmad N.Gany, Najah R. Hadi, Rihab H. Al-Mudhafar, Dhefaf H. Al-Mudhafar. Effect Of Topically Applied Melatonin And Their Combination With Minoxidil Solution For Enhancement Of Hair Growth In Male Mice. SRP. (2021), [cited March 30, 2021]; 12(1): 402-410. doi:10.31838/srp.2021.1.61 Harvard Style Hasanain H. Ali, Sarmad N.Gany, Najah R. Hadi, Rihab H. Al-Mudhafar, Dhefaf H. Al-Mudhafar (2021) Effect Of Topically Applied Melatonin And Their Combination With Minoxidil Solution For Enhancement Of Hair Growth In Male Mice. SRP, 12 (1), 402-410. doi:10.31838/srp.2021.1.61 Turabian Style Hasanain H. Ali, Sarmad N.Gany, Najah R. Hadi, Rihab H. Al-Mudhafar, Dhefaf H. Al-Mudhafar. 2021. Effect Of Topically Applied Melatonin And Their Combination With Minoxidil Solution For Enhancement Of Hair Growth In Male Mice. Systematic Reviews in Pharmacy, 12 (1), 402-410. doi:10.31838/srp.2021.1.61 Chicago Style Hasanain H. Ali, Sarmad N.Gany, Najah R. Hadi, Rihab H. Al-Mudhafar, Dhefaf H. Al-Mudhafar. "Effect Of Topically Applied Melatonin And Their Combination With Minoxidil Solution For Enhancement Of Hair Growth In Male Mice." Systematic Reviews in Pharmacy 12 (2021), 402-410. doi:10.31838/srp.2021.1.61 MLA (The Modern Language Association) Style Hasanain H. Ali, Sarmad N.Gany, Najah R. Hadi, Rihab H. Al-Mudhafar, Dhefaf H. Al-Mudhafar. "Effect Of Topically Applied Melatonin And Their Combination With Minoxidil Solution For Enhancement Of Hair Growth In Male Mice." Systematic Reviews in Pharmacy 12.1 (2021), 402-410. Print. doi:10.31838/srp.2021.1.61 APA (American Psychological Association) Style Hasanain H. Ali, Sarmad N.Gany, Najah R. Hadi, Rihab H. Al-Mudhafar, Dhefaf H. Al-Mudhafar (2021) Effect Of Topically Applied Melatonin And Their Combination With Minoxidil Solution For Enhancement Of Hair Growth In Male Mice. Systematic Reviews in Pharmacy, 12 (1), 402-410. doi:10.31838/srp.2021.1.61

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