Expression of Toll ? Like Receptor (TLR4) Gene in Type 2 Diabetic Normal Albuminuria, Micro Albuminuria and Macro Albuminuria Patients in Najaf Governorate ? Iraq

Abstract

Rawaa Behlul Al-Fatlawi, Abdullah Elttayef Jasim, Munther Abosaooda, Kifah Jabbar Al-Yaqoobi, Najah R. Hadi, Wasan Sami Hamid Shukur, Kareem Ghaly

TLR-4 receptor plays an important role in the pathogenesis of many inflammatory diseases like diabetes mellitus. The present study was designed to examine TLR-4 gene expression in Type 2 diabetes. Gene expression of TLR-4 the mean± SD of ΔCt (normalization Ct values) of each study group: The mean ±SD Ct value of TLR-4 cDNA amplification was (22.28± 0.36) Normo Albuminuria group, Microalbuminuria group was 20.28± 0.41). The Ct values of Macro Albuminuria group 21.51± 0.31). There was a significant difference in the mean Ct values between different groups, (p=0.0001). The mean Ct values of control (Normo Albuminuria) group were higher than the Macro and this in turn was higher than that of Micro groups the mean± SD of ΔCt (normalization Ct values) of each study group, for the Micro Albuminuria (3.04±2.5). For Macro Albuminuria group the mA significant difference was noticed between the study groups (p=0.001). Results of 2-ΔCt revealed significantly higher results for the Micro Albuminuria group from the other two groups (p=0.0001). 2-ΔCt for micro Albuminuria group (0.121). In the Macro Albuminuria group and Normo Albuminuria group a 2-ΔCt were (0.051), (0.029) respectively. There is a significantly increased expression of TLR-4 gene in macro and Micro Albuminuria groups as compared to Normoalbuminuria group.