Flavonoid Separation from different extracts of aerial parts of Convolvulus arvensis by HPLC and Screening of Anthelmintic Activity
Abstract
Objective: Convolvulus arvensis is one of the world’s top noxious weeds and is commonly known as field bindweed. The objective of research work was separation and identification of flavonoids and to check the anthelmintic activity of this plant.
Methods: The flavonoids were analyzed by High-Performance Liquid Chromatography (HPLC) method from alcoholic, hydroalcoholic and aqueous extract of aerial parts of Convolvulus arvensis . Acetonitrile:water (containing 0.05% Trifluoroacetic Acid (TFA) each) was used as mobile phase and isolation was done by using RP-18 column with UV variable wavelength detector (254 nm). Chromatogram of standards was compared with sample. Anthelmintic activity also was performed on prepared extracts of various concentrations.
Results: The presence of flavonoids like rutin, kaemferol, quercetin and quercetrin in crude extract was revealed. Significant anthelmintic activity in ethanolic extract was also reported.
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