Abstract

Maather Baqer Hussein Al-Harmooshee and Orass. M. Sh. Al-Taei

Background: RAD51 from the cluster genes which have a vital role in the pathogenesis of squamous cell carcinoma. Present study aimed to analysed polymorphisms of RAD5 single nucleotide 1(rs2619679, rs2928140 and rs1801320) and their relationship to breast cancer (BC) and ovarian cancer (OC) in Iraqi population. Methods: This study included, 35 females with BC, 35 females with OC, who were diagnostic histopathologicaly, and 30 healthy females as control. Three SNPs (rs2619679, rs2928140 and rs1801320) of RAD51 were selected for genotyping by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: Statistically differences were found in the distribution of AA and TT genotypes and A/T alleles for rs2619679 in BC (p<0.05) so GG and CC genotypes and G/C alleles for rs2928140. Whereas distribution of genotypes and alleles for rs1801320 not reflect significant differences (p>0.05) between BC and control but mutant genotype GG and mutant allele G appeared in highest frequency (42%, 57%) in BC. Statistically changes of RAD51 SNPs for OC were found in the incidence of AA and T alleles for rs2619679 (p<0.05) so, GG and CC genotypes and G/C alleles for rs2928140 but distribution of genotypes and alleles for rs1801320 not reproduce significant differences (p>0.05) between OC and control but mutant genotype GG and mutant allele G appeared in OC with highest rate (45%, 59%). Mutant genotype/allele of rs2619679 (TT/T), rs2928140(CC/C) and rs1801320 (GG/G) appeared as effective factors for cancer with acceptable rate for diagnosis of BC and OC. Conclusion: mutation in RAD51 SNPs (rs2619679, rs2928140 , rs1801320) associated with increases the possibility of BC and OC.

 

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Pubmed Style Maather Baqer Hussein Al-Harmooshee and Orass. M. Sh. Al-Taei. Germline Mutation of RAD51 Single Nucleotide Polymorphisms as Susceptibility Factor for Breast and Ovarian Cancer. SRP. 2020; 11(10): 100-108. doi:10.31838/srp.2020.10.17 Web Style Maather Baqer Hussein Al-Harmooshee and Orass. M. Sh. Al-Taei. Germline Mutation of RAD51 Single Nucleotide Polymorphisms as Susceptibility Factor for Breast and Ovarian Cancer. http://www.sysrevpharm.org/?mno=10190 [Access: March 28, 2021]. doi:10.31838/srp.2020.10.17 AMA (American Medical Association) Style Maather Baqer Hussein Al-Harmooshee and Orass. M. Sh. Al-Taei. Germline Mutation of RAD51 Single Nucleotide Polymorphisms as Susceptibility Factor for Breast and Ovarian Cancer. SRP. 2020; 11(10): 100-108. doi:10.31838/srp.2020.10.17 Vancouver/ICMJE Style Maather Baqer Hussein Al-Harmooshee and Orass. M. Sh. Al-Taei. Germline Mutation of RAD51 Single Nucleotide Polymorphisms as Susceptibility Factor for Breast and Ovarian Cancer. SRP. (2020), [cited March 28, 2021]; 11(10): 100-108. doi:10.31838/srp.2020.10.17 Harvard Style Maather Baqer Hussein Al-Harmooshee and Orass. M. Sh. Al-Taei (2020) Germline Mutation of RAD51 Single Nucleotide Polymorphisms as Susceptibility Factor for Breast and Ovarian Cancer. SRP, 11 (10), 100-108. doi:10.31838/srp.2020.10.17 Turabian Style Maather Baqer Hussein Al-Harmooshee and Orass. M. Sh. Al-Taei. 2020. Germline Mutation of RAD51 Single Nucleotide Polymorphisms as Susceptibility Factor for Breast and Ovarian Cancer. Systematic Reviews in Pharmacy, 11 (10), 100-108. doi:10.31838/srp.2020.10.17 Chicago Style Maather Baqer Hussein Al-Harmooshee and Orass. M. Sh. Al-Taei. "Germline Mutation of RAD51 Single Nucleotide Polymorphisms as Susceptibility Factor for Breast and Ovarian Cancer." Systematic Reviews in Pharmacy 11 (2020), 100-108. doi:10.31838/srp.2020.10.17 MLA (The Modern Language Association) Style Maather Baqer Hussein Al-Harmooshee and Orass. M. Sh. Al-Taei. "Germline Mutation of RAD51 Single Nucleotide Polymorphisms as Susceptibility Factor for Breast and Ovarian Cancer." Systematic Reviews in Pharmacy 11.10 (2020), 100-108. Print. doi:10.31838/srp.2020.10.17 APA (American Psychological Association) Style Maather Baqer Hussein Al-Harmooshee and Orass. M. Sh. Al-Taei (2020) Germline Mutation of RAD51 Single Nucleotide Polymorphisms as Susceptibility Factor for Breast and Ovarian Cancer. Systematic Reviews in Pharmacy, 11 (10), 100-108. doi:10.31838/srp.2020.10.17