Abstract

Ameen Waleed Qassim, Ashraf Saad Rasheed, Salsal Kamal Abdulrahman.

The hydrophilic interaction chromatography (HILIC) coupled with UV detection was employed to study retention behaviour and separation of genistein. The genistein is a compound of the isoflavone family found in natural resources in various plants, including herbs, tea, bee products and legumes such as soybeans. A simple, fast and efficient HILIC-UV method was developed for the quantitative and qualitative purposes of genistein in fenugreek seeds, olive leaf, and propolis. The best separation was achieved using a gradient elution of acetonitrile and acetate buffer on ZIC-HILIC column from Merck SeQuant (100 mm×4.6 mm I.D., 5μm) at 35 °C. The genistein was identified with a UV detector at 260 nm. The result demonstrates that the linear range 0.05-5 µgmL-1, high precision (RSD% < 0.5%), and excellent validated values for both LOD (0.0047 µgmL-1) and LOQ (0.0142 µgmL-1).