Immunoassay and Genotyping of Hepatitis C virus in Ramadi City
Abstract
Noor Naji Al-Hayani, Huda Rafaa Al-Alwani, Muntaha M. Hasan Al-alouci.
The study aimed to detect the prevalence of hepatitis C virus in two groups of population, hemodialysis patients and blood donors by different diagnostic methods and also to identify the dominant genotype of this virus in our area. All the samples that collected from blood donors and hemodialysis patients were tested by Rapid test and ELISA technique to detect the positive cases of hepatitis C virus. Only the positive samples were tested by real time PCR for genotyping. 75 hemodialysis patients 27 were infected with HCV and only 13 were blood donors from total number 4560. The total number of positive HCV were 40 samples, all the 40 cases were positive by Rapid and ELISA tests while only 38 were positive by real time PCR. The genotypes of this virus within our cases include genotype 4 (26) and genotype 1a (12). This study concludes that rapid test, ELISA and real time PCR are highly sensitive in detection of this virus. The dominant genotype in our area is genotype 4.
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Pubmed Style Al-Hayani NN, Al-Alwani HR, Al-alouci MMH. Immunoassay and Genotyping of Hepatitis C virus in Ramadi City. SRP. 2021; 12(1): 1088-1093. doi:10.31838/srp.2021.1.151 Web Style Al-Hayani NN, Al-Alwani HR, Al-alouci MMH. Immunoassay and Genotyping of Hepatitis C virus in Ramadi City. http://www.sysrevpharm.org/?mno=47293 [Access: March 31, 2021]. doi:10.31838/srp.2021.1.151 AMA (American Medical Association) Style Al-Hayani NN, Al-Alwani HR, Al-alouci MMH. Immunoassay and Genotyping of Hepatitis C virus in Ramadi City. SRP. 2021; 12(1): 1088-1093. doi:10.31838/srp.2021.1.151 Vancouver/ICMJE Style Al-Hayani NN, Al-Alwani HR, Al-alouci MMH. Immunoassay and Genotyping of Hepatitis C virus in Ramadi City. SRP. (2021), [cited March 31, 2021]; 12(1): 1088-1093. doi:10.31838/srp.2021.1.151 Harvard Style Al-Hayani, N. N., Al-Alwani, . H. R. & Al-alouci, . M. M. H. (2021) Immunoassay and Genotyping of Hepatitis C virus in Ramadi City. SRP, 12 (1), 1088-1093. doi:10.31838/srp.2021.1.151 Turabian Style Al-Hayani, Noor Naji, Huda Rafaa Al-Alwani, and Muntaha M. Hasan Al-alouci. 2021. Immunoassay and Genotyping of Hepatitis C virus in Ramadi City. Systematic Reviews in Pharmacy, 12 (1), 1088-1093. doi:10.31838/srp.2021.1.151 Chicago Style Al-Hayani, Noor Naji, Huda Rafaa Al-Alwani, and Muntaha M. Hasan Al-alouci. "Immunoassay and Genotyping of Hepatitis C virus in Ramadi City." Systematic Reviews in Pharmacy 12 (2021), 1088-1093. doi:10.31838/srp.2021.1.151 MLA (The Modern Language Association) Style Al-Hayani, Noor Naji, Huda Rafaa Al-Alwani, and Muntaha M. Hasan Al-alouci. "Immunoassay and Genotyping of Hepatitis C virus in Ramadi City." Systematic Reviews in Pharmacy 12.1 (2021), 1088-1093. Print. doi:10.31838/srp.2021.1.151 APA (American Psychological Association) Style Al-Hayani, N. N., Al-Alwani, . H. R. & Al-alouci, . M. M. H. (2021) Immunoassay and Genotyping of Hepatitis C virus in Ramadi City. Systematic Reviews in Pharmacy, 12 (1), 1088-1093. doi:10.31838/srp.2021.1.151 |