Influence of a Pigment Protein Fraction from Chlorella vulgaris Isolated in Indonesia on ?-Actin and MHC-1 Response to Viral Infected Humpback Grouper
Abstract
Uun Yanuhar, Muhammad Musa, Diana Arfiati, Qomariyatus Sholihah, Ach. Khumaid, Nico Rahman Caesar.
Study aims to show the capability of Pigment-Protein Fractions extracted from C. vulgaris for treating the infection of Viral Nervous Necrosis (VNN) in Humpback grouper. Study was conducted by isolation and culture C. vulgaris after identified using 16 sRNA. PPF was extracted and analyzed using the SDS-page. Fish treatment were divided in four groups: (A) normal fish/control), (B) PPF administration into fish, (C) VNN induction into fish, and (D) both VNN and PPF induction into fish. ï¢-Actin and MHC-1 response were evaluated using ImmunoRatio (IR) analysis. The results show that microalgae that local isolated from Indonesia is confirm as C. vulgaris with nucleotide sequences of 424 bp. Extracted PPF of C. vulgaris show a 33 kDa of protein molecule weight. Treatment in group B, C, and D able to increases the MHC-1 expression as much as 26.1%, 35%, and 66.9%, respectively. PCR quantification results in all PPF test treatments showed the presence of B-actin expression. Histopathology observation on eye tissue indicates occurred Necrosis (N) in group B, Vacuolation (V) and Necrosis (N) in group C. In group D, there are Vacuolation (V) and Necrosis (N) but less tissue damage compare than group B. It is indicates that C. vulgaris have a positive influence on the immune response of groupers (C. altivelis) after infected by VNN.
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| Pubmed Style Yanuhar U, Musa M, Arfiati D, Sholihah Q, Khumaid A, Caesar NR. Influence of a Pigment Protein Fraction from Chlorella vulgaris Isolated in Indonesia on ?-Actin and MHC-1 Response to Viral Infected Humpback Grouper. SRP. 2020; 11(7): 352-362. doi:10.31838/srp.2020.7.54 Web Style Yanuhar U, Musa M, Arfiati D, Sholihah Q, Khumaid A, Caesar NR. Influence of a Pigment Protein Fraction from Chlorella vulgaris Isolated in Indonesia on ?-Actin and MHC-1 Response to Viral Infected Humpback Grouper. http://www.sysrevpharm.org/?mno=127610 [Access: March 29, 2021]. doi:10.31838/srp.2020.7.54 AMA (American Medical Association) Style Yanuhar U, Musa M, Arfiati D, Sholihah Q, Khumaid A, Caesar NR. Influence of a Pigment Protein Fraction from Chlorella vulgaris Isolated in Indonesia on ?-Actin and MHC-1 Response to Viral Infected Humpback Grouper. SRP. 2020; 11(7): 352-362. doi:10.31838/srp.2020.7.54 Vancouver/ICMJE Style Yanuhar U, Musa M, Arfiati D, Sholihah Q, Khumaid A, Caesar NR. Influence of a Pigment Protein Fraction from Chlorella vulgaris Isolated in Indonesia on ?-Actin and MHC-1 Response to Viral Infected Humpback Grouper. SRP. (2020), [cited March 29, 2021]; 11(7): 352-362. doi:10.31838/srp.2020.7.54 Harvard Style Yanuhar, U., Musa, . M., Arfiati, . D., Sholihah, . Q., Khumaid, . A. & Caesar, . N. R. (2020) Influence of a Pigment Protein Fraction from Chlorella vulgaris Isolated in Indonesia on ?-Actin and MHC-1 Response to Viral Infected Humpback Grouper. SRP, 11 (7), 352-362. doi:10.31838/srp.2020.7.54 Turabian Style Yanuhar, Uun, Muhammad Musa, Diana Arfiati, Qomariyatus Sholihah, Ach. Khumaid, and Nico Rahman Caesar. 2020. Influence of a Pigment Protein Fraction from Chlorella vulgaris Isolated in Indonesia on ?-Actin and MHC-1 Response to Viral Infected Humpback Grouper. Systematic Reviews in Pharmacy, 11 (7), 352-362. doi:10.31838/srp.2020.7.54 Chicago Style Yanuhar, Uun, Muhammad Musa, Diana Arfiati, Qomariyatus Sholihah, Ach. Khumaid, and Nico Rahman Caesar. "Influence of a Pigment Protein Fraction from Chlorella vulgaris Isolated in Indonesia on ?-Actin and MHC-1 Response to Viral Infected Humpback Grouper." Systematic Reviews in Pharmacy 11 (2020), 352-362. doi:10.31838/srp.2020.7.54 MLA (The Modern Language Association) Style Yanuhar, Uun, Muhammad Musa, Diana Arfiati, Qomariyatus Sholihah, Ach. Khumaid, and Nico Rahman Caesar. "Influence of a Pigment Protein Fraction from Chlorella vulgaris Isolated in Indonesia on ?-Actin and MHC-1 Response to Viral Infected Humpback Grouper." Systematic Reviews in Pharmacy 11.7 (2020), 352-362. Print. doi:10.31838/srp.2020.7.54 APA (American Psychological Association) Style Yanuhar, U., Musa, . M., Arfiati, . D., Sholihah, . Q., Khumaid, . A. & Caesar, . N. R. (2020) Influence of a Pigment Protein Fraction from Chlorella vulgaris Isolated in Indonesia on ?-Actin and MHC-1 Response to Viral Infected Humpback Grouper. Systematic Reviews in Pharmacy, 11 (7), 352-362. doi:10.31838/srp.2020.7.54 |
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