Morphological Condition of the Large Intestine in Chronic Intoxication with Pesticides Against the Background of Diabetes Mellitus

Abstract

TUGANBAYEVA ALIYA, BEGIMBEKOVA LAZZAT, ALIYEVA ELNARA, OSPANOVA AKMARAL, UTEPOVA RAIKHAN, MUZDYBAYEVA GAUKHAR.

This article presents experimental research and study of the peculiarities of alternative and reactive changes in the colon during prolonged exposure to the most widely used pesticides of various chemical groups (copper-containing, organochlorine, organophosphate) against the background of diabetes mellitus. The aim of the study was to identify morphological changes in the microcirculatory bed and tissue structures of the colon in chronic poisoning with pesticides "Neoron" and "Sumi-alpha" on the background of diabetes mellitus. Material and methods of research. The object of the study was the Ambassador of 68 white laboratory rats-males with an initial mass of 80-150 grams, who were in normal vivarium conditions. Modeling of alloxan diabetes was reproduced by a single intraperitoneal injection of 1.0 ml/100 g of alloxan in a phosphate-citrate buffer at the rate of 11 mg /100 g of mass. This study is retrospective, with historical data coverage, and prospective preclinical, experimental. Statistical processing of the obtained results was performed using standard student T-test methods to assess the reliability of differences using the SPSS-21 version program. The average values are represented as M±SD. Differences at p<0.05 were considered reliable. Results. Long-term periods are characterized by an increase in atrophic processes, leading to a noticeable thinning of the wall throughout. There were pronounced phenomena of crypt polymorphism, and glued crypts were found in some areas. In some places, as a result of massive desquamation of the epithelium, there was a Baring of mucosal areas, as well as ulcerative defects, around which there was a massive infiltration of leukocytes and macrophages. In such areas, the area of non-vascular zones increases, which worsens the course of pathomorphological processes. Conclusion. The data obtained by us indicate that in the early periods (3-15 days) after acute priming with pesticides "Neoron" and "Sumi-alpha", animals show pronounced inflammatory and destructive changes in all layers of the colon wall, especially the mucosa.