Abstract

Awatif H. Issa, Abdulelah A. Almayah, Hanaa k. Ibrahim.

Histamine- producing bacteria may be associated with much irritable bowel disease. The present study was feces samples for histamine producing bacteria (HPB) isolation. Detection was depended on Niven´s medium that out of 30 bacterial samples, only 21 isolates were positive for histamine production. The bacterial species were identified by Vitek2 –compact results. E.coli species were selected for present study. Vitek2 –compact device was used to study the sensitivity of bacterial isolates against 16 antibiotic. Most E.coli species were resistant to beta- Lactamaes (Ampicillin/Sulbactam Ampicillin and Ciprofloxacin), and sensitive to Trimethoprim (100%. The study showed that E.coli have histidine decarboxylase enzyme that has the ability to produce histamine and the occurrence of the disease, which is considered a virulence factor. The concentration of histamine was detected by HPLC technique (21.01 µg/ml. There are various factors that affect the bacterial production of histamine were detected include pH, salt and temperature. E.coli proportion to the concentration of histamine in pH (6) 21.41µl/mg and less at a temperature 40°C was 4.82 µl/mg.