NK Cells Induced by Ethanolic Nigella sativa Extract Inhibits Proliferation of Retinoblastoma Y79 Cell Line Through Cyclin D1 Pathway
Abstract
Alfa Sylvestris, Djanggan Sargowo, Mohammad Saifur Rohman, Hidayat Sujuti
Nigella sativa can increase the immunomodulatory function of NK cells. This present study aimed to analyze the anti-proliferative effect of NK cells induced by Nigella sativa ethanolic extract on Y79 retinoblastoma cell line through the cyclin D1 pathway. Flow cytometry was used on co-cultured Y79 cells with NK cells induced by Nigella sativa ethanolic extract at various doses: 0, 25, and 100 μg/ml with incubation for 24 hours and a moi (multiplicity of infection) ratio of 1: 1 and 1: 5. ANOVA results showed a significant correlation (p <0.001) between dose and moi interactions with cyclin D1 expression, and Y79 proliferation. The Tukey Post Hoc results showed the lowest cyclin D1 expression at a dose of 100 μg/ml and moi 1: 5 of 6.75 ± 0.30. Whereas in the Tukey Post Hoc results, the proliferation showed decreased proliferation at a dose of 100 μg/ml with 1: 1 and 1: 5 moi. Path analysis showed the relationship between the induction of Nigella sativa ethanolic extract on NK cells with significant and negative cyclin D1 expression. Meanwhile, the path analysis relationship between cyclin D1 expression and proliferation showed a significantly positive relationship. So it is proven that the NK cell anti-proliferation pathway after induced by the ethanolic Nigella sativa extract is indeed through the cyclin D1 pathway. Cyclin D1 is one of the potent proliferation markers, a decrease in cyclin D1 expression will also be followed by a decrease in the proliferation of Y79 retinoblastoma cancer cells.
| How to Cite this Article |
| Pubmed Style Sylvestris A, Sargowo D, Rohman MS, Sujuti H. NK Cells Induced by Ethanolic Nigella sativa Extract Inhibits Proliferation of Retinoblastoma Y79 Cell Line Through Cyclin D1 Pathway. SRP. 2021; 12(1): 1545-1550. doi:10.31838/srp.2021.1.217 Web Style Sylvestris A, Sargowo D, Rohman MS, Sujuti H. NK Cells Induced by Ethanolic Nigella sativa Extract Inhibits Proliferation of Retinoblastoma Y79 Cell Line Through Cyclin D1 Pathway. http://www.sysrevpharm.org/?mno=52421 [Access: March 30, 2021]. doi:10.31838/srp.2021.1.217 AMA (American Medical Association) Style Sylvestris A, Sargowo D, Rohman MS, Sujuti H. NK Cells Induced by Ethanolic Nigella sativa Extract Inhibits Proliferation of Retinoblastoma Y79 Cell Line Through Cyclin D1 Pathway. SRP. 2021; 12(1): 1545-1550. doi:10.31838/srp.2021.1.217 Vancouver/ICMJE Style Sylvestris A, Sargowo D, Rohman MS, Sujuti H. NK Cells Induced by Ethanolic Nigella sativa Extract Inhibits Proliferation of Retinoblastoma Y79 Cell Line Through Cyclin D1 Pathway. SRP. (2021), [cited March 30, 2021]; 12(1): 1545-1550. doi:10.31838/srp.2021.1.217 Harvard Style Sylvestris, A., Sargowo, . D., Rohman, . M. S. & Sujuti, . H. (2021) NK Cells Induced by Ethanolic Nigella sativa Extract Inhibits Proliferation of Retinoblastoma Y79 Cell Line Through Cyclin D1 Pathway. SRP, 12 (1), 1545-1550. doi:10.31838/srp.2021.1.217 Turabian Style Sylvestris, Alfa, Djanggan Sargowo, Mohammad Saifur Rohman, and Hidayat Sujuti. 2021. NK Cells Induced by Ethanolic Nigella sativa Extract Inhibits Proliferation of Retinoblastoma Y79 Cell Line Through Cyclin D1 Pathway. Systematic Reviews in Pharmacy, 12 (1), 1545-1550. doi:10.31838/srp.2021.1.217 Chicago Style Sylvestris, Alfa, Djanggan Sargowo, Mohammad Saifur Rohman, and Hidayat Sujuti. "NK Cells Induced by Ethanolic Nigella sativa Extract Inhibits Proliferation of Retinoblastoma Y79 Cell Line Through Cyclin D1 Pathway." Systematic Reviews in Pharmacy 12 (2021), 1545-1550. doi:10.31838/srp.2021.1.217 MLA (The Modern Language Association) Style Sylvestris, Alfa, Djanggan Sargowo, Mohammad Saifur Rohman, and Hidayat Sujuti. "NK Cells Induced by Ethanolic Nigella sativa Extract Inhibits Proliferation of Retinoblastoma Y79 Cell Line Through Cyclin D1 Pathway." Systematic Reviews in Pharmacy 12.1 (2021), 1545-1550. Print. doi:10.31838/srp.2021.1.217 APA (American Psychological Association) Style Sylvestris, A., Sargowo, . D., Rohman, . M. S. & Sujuti, . H. (2021) NK Cells Induced by Ethanolic Nigella sativa Extract Inhibits Proliferation of Retinoblastoma Y79 Cell Line Through Cyclin D1 Pathway. Systematic Reviews in Pharmacy, 12 (1), 1545-1550. doi:10.31838/srp.2021.1.217 |
Abstract
PDF
How to cite this article
Related Records