Abstract

Mohammed Abbas Fadhil, Enas Jawad Kadhim

The study provides the first comprehensive research done in Iraq to study the phytochemicals and the methods of extraction and separation of some phenolic compounds from carthamus tinctorius grown naturally in Iraq. Preliminary phytochemical screening of Carthamus tinctorious crude extract was revealed the presence of flavonoids, phenols, terpenoids, cardiac glycoside and tannin. Leaves parts of plant was extracted by hot extraction methods using 90% ethanol and fractionation was done to separate the active constituents according to difference in polarities using pet. Ether, chloroform, ethyl acetate and n-butanol. The n-butanol, fraction was used for detection and separation of flavonoids and phenolic acids by TLC, and HPLC. Flavonoid (apigenin), and the Phenolic acid (gallic acid) were isolated and purified by preparative thin layer chromatography. The isolated compounds were subjected to various chemical, chromatographic and spectral analytical techniques for their identification such as TLC, HPLC, and FTIR.