Abstract

Iif Hanifa Nurrosyidah, Isnaeni Isnaeni, Ni Made Mertaniasih

The present study aimed to characterize the potency of indigenous lactic acid bacteria (LAB) isolated from red passion fruit (Passiflora edulis Sims) as probiotic and evaluate their antibiotic sensitivity. More than 50 suspected LAB was isolated by selective medium of Man Rogosa Sharpe (MRS) agar. Identification of LAB was determined through the morphological, phenotype, and biochemical analysis. Ten isolates (MM1-MM10) were identified as LAB by further analysis of 16s rRNA. However only three isolates (MM1, MM2 and MM3) was indicated having probiotic characteristic; able to survive at low pH media, tolerance to salt and phenol. Three isolates (MM1, MM2, and MM3) were identified 16s rRNa with the results; Bacillus subtillis (MM1), Bacillus wiedmannii (MM2), and Bacillus cereus (MM3). In addition, those isolates also showed resistance against two antibiotics: erythromycin and vancomycin at 5 μg/mL and 2.5 μg/mL, respectively. Both concentrations were higher than minimum inhibitory concentration (MIC). MM1 showed higher susceptibility followed by MM2 and MM3 isolates. Compatibility of isolates (MM1, MM2, and MM3) has been investigated and they are compatible. Thus, red passion fruit can be considered as source of probiotic which resistant to pathogens and antibiotics