Screening the frequency of panton-valentine leukocidin (pvl) gene between methicillin resistant Staphylococcus aureus isolated from diabetic foot patients in Al-Basrah governorate, south of Iraq
Abstract
Tahrir H. Gadban, Saad S. Al-Amara, Hanadi A. Jasim.
Staphylococcus aureus with methicillin resistant is an important pathogen associated with diabetic foot ulcers (DFUs).The (pvl) gene is considered a marker for MRSA that used widely as acquired in the population, it's responsible for deep dermal infections and soft tissue. The current research aimed to establish the panton-valentine leukocidin (pvl) frequency of gene between MRSA isolated from infected DFUs individuals in Al-Basrah governorate, south of Iraq.
Methods:
One hundred fifty swab sample from diabetic foot ulcers (DFUs) patients who admitted to two local hospitals in Basrah governorate.The S. aureus isolates were identified by criteria of growth on both blood agar and mannitol salt agar, and then it tested for coagulase test. The isolates confirmed as S.aureus by using the vitek®2 system for identifying the Staphylococcus aureus. MRSA had been confirmed by testing the isolates against oxacillin and cefoxitin disc.The 16S rRNA was used to confirm the identification of S.aureus isolates.the PCR was used to detect the production of pvl genes.
Results:
Out of 150 swab samples only 21 isolates which include 18(85.7%) isolates was identified as S. aureus, the other 3(14.3%) isolate was identified as Staphylococcus spp. The result of vitek ® 2 showed all 18(100%)isolates were identified as S. aureus. On the other hand all (18) S. aureus isolates given the inhibitory resistant criteria for oxacillin disc 1µg and cefoxitin disc 30 µg, these isolates classified as MRSA. Furthermore, the result of amplifying 16S rRNA was given the positive result to identify S.aureus . MRSA was found in all isolates, the 18 (100 %) were pvl positive.
Conclusion:
The frequency of pvl gene found in all MRSA isolates in this study. It can be used as community acquired-MRSA marker. The PCR analysis effective in detecting pvl & mecA genes
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| Pubmed Style Tahrir H. Gadban, Saad S. Al-Amara, Hanadi A. Jasim. Screening the frequency of panton-valentine leukocidin (pvl) gene between methicillin resistant Staphylococcus aureus isolated from diabetic foot patients in Al-Basrah governorate, south of Iraq. SRP. 2020; 11(11): 285-290. doi:10.31838/srp.2020.11.42 Web Style Tahrir H. Gadban, Saad S. Al-Amara, Hanadi A. Jasim. Screening the frequency of panton-valentine leukocidin (pvl) gene between methicillin resistant Staphylococcus aureus isolated from diabetic foot patients in Al-Basrah governorate, south of Iraq. http://www.sysrevpharm.org/?mno=9844 [Access: March 30, 2021]. doi:10.31838/srp.2020.11.42 AMA (American Medical Association) Style Tahrir H. Gadban, Saad S. Al-Amara, Hanadi A. Jasim. Screening the frequency of panton-valentine leukocidin (pvl) gene between methicillin resistant Staphylococcus aureus isolated from diabetic foot patients in Al-Basrah governorate, south of Iraq. SRP. 2020; 11(11): 285-290. doi:10.31838/srp.2020.11.42 Vancouver/ICMJE Style Tahrir H. Gadban, Saad S. Al-Amara, Hanadi A. Jasim. Screening the frequency of panton-valentine leukocidin (pvl) gene between methicillin resistant Staphylococcus aureus isolated from diabetic foot patients in Al-Basrah governorate, south of Iraq. SRP. (2020), [cited March 30, 2021]; 11(11): 285-290. doi:10.31838/srp.2020.11.42 Harvard Style Tahrir H. Gadban, Saad S. Al-Amara, Hanadi A. Jasim (2020) Screening the frequency of panton-valentine leukocidin (pvl) gene between methicillin resistant Staphylococcus aureus isolated from diabetic foot patients in Al-Basrah governorate, south of Iraq. SRP, 11 (11), 285-290. doi:10.31838/srp.2020.11.42 Turabian Style Tahrir H. Gadban, Saad S. Al-Amara, Hanadi A. Jasim. 2020. Screening the frequency of panton-valentine leukocidin (pvl) gene between methicillin resistant Staphylococcus aureus isolated from diabetic foot patients in Al-Basrah governorate, south of Iraq. Systematic Reviews in Pharmacy, 11 (11), 285-290. doi:10.31838/srp.2020.11.42 Chicago Style Tahrir H. Gadban, Saad S. Al-Amara, Hanadi A. Jasim. "Screening the frequency of panton-valentine leukocidin (pvl) gene between methicillin resistant Staphylococcus aureus isolated from diabetic foot patients in Al-Basrah governorate, south of Iraq." Systematic Reviews in Pharmacy 11 (2020), 285-290. doi:10.31838/srp.2020.11.42 MLA (The Modern Language Association) Style Tahrir H. Gadban, Saad S. Al-Amara, Hanadi A. Jasim. "Screening the frequency of panton-valentine leukocidin (pvl) gene between methicillin resistant Staphylococcus aureus isolated from diabetic foot patients in Al-Basrah governorate, south of Iraq." Systematic Reviews in Pharmacy 11.11 (2020), 285-290. Print. doi:10.31838/srp.2020.11.42 APA (American Psychological Association) Style Tahrir H. Gadban, Saad S. Al-Amara, Hanadi A. Jasim (2020) Screening the frequency of panton-valentine leukocidin (pvl) gene between methicillin resistant Staphylococcus aureus isolated from diabetic foot patients in Al-Basrah governorate, south of Iraq. Systematic Reviews in Pharmacy, 11 (11), 285-290. doi:10.31838/srp.2020.11.42 |
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