open-access
25+ Million Readerbase Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Citations Report
image image image image
Google Scholar citation report
Citations : 25586

Systematic Reviews in Pharmacy received 25586 citations as per google scholar report

Flyer image
Systematic Reviews in Pharmacy peer review process verified at publons
Flyer image

Indexed In

The Effect of Cobalt (II) Chloride in the Viability Percentage and the Induced Hypoxia Inducible Factor -1α of Human Adipose Mesenchymal Stem Cells (HAMSCs): An In Vitro Study

Abstract

Alexander Patera Nugraha, Eric Priyo Prasetyo, Mefina Kuntjoro, Igo Syaiful Ihsan, Aristika Dinaryanti, Helen Susilowati, Eryk Hendrianto, Ida Bagus Narmada, Diah Savitri Ernawati, Andreas Pratama Nugraha, Fedik Abdul Rantam.

Introduction: The preconditioning of HAMSCs using Cobalt (II) Chloride (CoCl2) as a hypoxia mimicking agent (HMA) may provide the adaptive state of HAMSCs.
Objective: To examine the effect of CoCl2 in the viability percentage and the induced Hypoxia Inducible Factor-1α (HIF-1α) expression of HAMSCs in vitro.
Methods: HAMSCs were thawed, cultured, and then sub-cultured from the laboratory cell stock. The Immunocytochemistry (ICC) methods with Fluorescein isothiocyanate (FITC) antibody labelling kit was used to validate the HAMSCs employing its Cluster of Differentiation (CD) expression such as CD73(+), CD105(+) and CD45(-). HAMSCs were sub-cultured and then co-cultivated with or without CoCl2 in the different concentration (200ï­mol, 150ï­mol, 100ï­mol, 75ï­mol, 50ï­mol, 25ï­mol) for 24 hours. The cytotoxicity test was conducted by utilizing tetrazolium dye MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. The formazan crystal was then observed, and the cell viability percentage was calculated. In examining CoCl2 induced hypoxia in HAMSCs, the expression of HIF-1α applied ICC-FITC in vitro. Analysis of Variance (ANOVA) was performed to analyze the obtained data statistically (p<0.05).
Results: HAMSCs express CD73 and C105 positively, while HAMSCs expressed CD45 negatively. There is no significant different in cell viability percentage of HAMCs between groups with different concentrations of CoCl2 (p=0.99; p>0.05). CoCl2 successfully induces the hypoxia confirmed by HIF-1α positive expression of HAMSCs after 24 hours incubation in vitro.
Conclusion: CoCl2 does not affect the viability percentage of HAMSCs and it can be used as a biocompatible HMA in HAMSCs in vitro.

How to Cite this Article
Pubmed Style

Alexander Patera Nugraha, Eric Priyo Prasetyo, Mefina Kuntjoro, Igo Syaiful Ihsan, Aristika Dinaryanti, Helen Susilowati, Eryk Hendrianto, Ida Bagus Narmada, Diah Savitri Ernawati, Andreas Pratama Nugraha, Fedik Abdul Rantam. The Effect of Cobalt (II) Chloride in the Viability Percentage and the Induced Hypoxia Inducible Factor -1α of Human Adipose Mesenchymal Stem Cells (HAMSCs): An In Vitro Study. SRP. 2020; 11(6): 308-314. doi:10.31838/srp.2020.6.49

Web Style

Alexander Patera Nugraha, Eric Priyo Prasetyo, Mefina Kuntjoro, Igo Syaiful Ihsan, Aristika Dinaryanti, Helen Susilowati, Eryk Hendrianto, Ida Bagus Narmada, Diah Savitri Ernawati, Andreas Pratama Nugraha, Fedik Abdul Rantam. The Effect of Cobalt (II) Chloride in the Viability Percentage and the Induced Hypoxia Inducible Factor -1α of Human Adipose Mesenchymal Stem Cells (HAMSCs): An In Vitro Study. http://www.sysrevpharm.org/?mno=113866 [Access: March 29, 2021]. doi:10.31838/srp.2020.6.49

AMA (American Medical Association) Style

Alexander Patera Nugraha, Eric Priyo Prasetyo, Mefina Kuntjoro, Igo Syaiful Ihsan, Aristika Dinaryanti, Helen Susilowati, Eryk Hendrianto, Ida Bagus Narmada, Diah Savitri Ernawati, Andreas Pratama Nugraha, Fedik Abdul Rantam. The Effect of Cobalt (II) Chloride in the Viability Percentage and the Induced Hypoxia Inducible Factor -1α of Human Adipose Mesenchymal Stem Cells (HAMSCs): An In Vitro Study. SRP. 2020; 11(6): 308-314. doi:10.31838/srp.2020.6.49


Vancouver/ICMJE Style

Alexander Patera Nugraha, Eric Priyo Prasetyo, Mefina Kuntjoro, Igo Syaiful Ihsan, Aristika Dinaryanti, Helen Susilowati, Eryk Hendrianto, Ida Bagus Narmada, Diah Savitri Ernawati, Andreas Pratama Nugraha, Fedik Abdul Rantam. The Effect of Cobalt (II) Chloride in the Viability Percentage and the Induced Hypoxia Inducible Factor -1α of Human Adipose Mesenchymal Stem Cells (HAMSCs): An In Vitro Study. SRP. (2020), [cited March 29, 2021]; 11(6): 308-314. doi:10.31838/srp.2020.6.49


Harvard Style

Alexander Patera Nugraha, Eric Priyo Prasetyo, Mefina Kuntjoro, Igo Syaiful Ihsan, Aristika Dinaryanti, Helen Susilowati, Eryk Hendrianto, Ida Bagus Narmada, Diah Savitri Ernawati, Andreas Pratama Nugraha, Fedik Abdul Rantam (2020) The Effect of Cobalt (II) Chloride in the Viability Percentage and the Induced Hypoxia Inducible Factor -1α of Human Adipose Mesenchymal Stem Cells (HAMSCs): An In Vitro Study. SRP, 11 (6), 308-314. doi:10.31838/srp.2020.6.49


Turabian Style

Alexander Patera Nugraha, Eric Priyo Prasetyo, Mefina Kuntjoro, Igo Syaiful Ihsan, Aristika Dinaryanti, Helen Susilowati, Eryk Hendrianto, Ida Bagus Narmada, Diah Savitri Ernawati, Andreas Pratama Nugraha, Fedik Abdul Rantam. 2020. The Effect of Cobalt (II) Chloride in the Viability Percentage and the Induced Hypoxia Inducible Factor -1α of Human Adipose Mesenchymal Stem Cells (HAMSCs): An In Vitro Study. Systematic Reviews in Pharmacy, 11 (6), 308-314. doi:10.31838/srp.2020.6.49


Chicago Style

Alexander Patera Nugraha, Eric Priyo Prasetyo, Mefina Kuntjoro, Igo Syaiful Ihsan, Aristika Dinaryanti, Helen Susilowati, Eryk Hendrianto, Ida Bagus Narmada, Diah Savitri Ernawati, Andreas Pratama Nugraha, Fedik Abdul Rantam. "The Effect of Cobalt (II) Chloride in the Viability Percentage and the Induced Hypoxia Inducible Factor -1α of Human Adipose Mesenchymal Stem Cells (HAMSCs): An In Vitro Study." Systematic Reviews in Pharmacy 11 (2020), 308-314. doi:10.31838/srp.2020.6.49


MLA (The Modern Language Association) Style

Alexander Patera Nugraha, Eric Priyo Prasetyo, Mefina Kuntjoro, Igo Syaiful Ihsan, Aristika Dinaryanti, Helen Susilowati, Eryk Hendrianto, Ida Bagus Narmada, Diah Savitri Ernawati, Andreas Pratama Nugraha, Fedik Abdul Rantam. "The Effect of Cobalt (II) Chloride in the Viability Percentage and the Induced Hypoxia Inducible Factor -1α of Human Adipose Mesenchymal Stem Cells (HAMSCs): An In Vitro Study." Systematic Reviews in Pharmacy 11.6 (2020), 308-314. Print. doi:10.31838/srp.2020.6.49


APA (American Psychological Association) Style

Alexander Patera Nugraha, Eric Priyo Prasetyo, Mefina Kuntjoro, Igo Syaiful Ihsan, Aristika Dinaryanti, Helen Susilowati, Eryk Hendrianto, Ida Bagus Narmada, Diah Savitri Ernawati, Andreas Pratama Nugraha, Fedik Abdul Rantam (2020) The Effect of Cobalt (II) Chloride in the Viability Percentage and the Induced Hypoxia Inducible Factor -1α of Human Adipose Mesenchymal Stem Cells (HAMSCs): An In Vitro Study. Systematic Reviews in Pharmacy, 11 (6), 308-314. doi:10.31838/srp.2020.6.49

ARTICLE TOOLS
ARTICLE TOOLS
Submit Article
Login
Register
Scan Code
Most Viewed Articles
  • Dental Development between Assisted Reproductive Therapy (Art) and Natural Conceived Children: A Comparative Pilot Study Norzaiti Mohd Kenali, Naimah Hasanah Mohd Fathil, Norbasyirah Bohari, Ahmad Faisal Ismail, Roszaman Ramli SRP. 2020; 11(1): 01-06 » doi: 10.5530/srp.2020.1.01
  • Psychometric properties of the World Health Organization Quality of life instrument, short form: Validity in the Vietnamese healthcare context Trung Quang Vo*, Bao Tran Thuy Tran, Ngan Thuy Nguyen, Tram ThiHuyen Nguyen, Thuy Phan Chung Tran SRP. 2020; 11(1): 14-22 » doi: 10.5530/srp.2019.1.3
  • A Review of Pharmacoeconomics: the key to “Healthcare for All” Hasamnis AA, Patil SS, Shaik Imam, Narendiran K SRP. 2019; 10(1): s40-s42 » doi: 10.5530/srp.2019.1s.21
  • Deuterium Depleted Water as an Adjuvant in Treatment of Cancer Anton Syroeshkin, Olga Levitskaya, Elena Uspenskaya, Tatiana Pleteneva, Daria Romaykina, Daria Ermakova SRP. 2019; 10(1): 112-117 » doi: 10.5530/srp.2019.1.19
Most Downloaded
  • Dental Development between Assisted Reproductive Therapy (Art) and Natural Conceived Children: A Comparative Pilot Study Norzaiti Mohd Kenali, Naimah Hasanah Mohd Fathil, Norbasyirah Bohari, Ahmad Faisal Ismail, Roszaman Ramli SRP. 2020; 11(1): 01-06 » doi: 10.5530/srp.2020.1.01
  • Manilkara zapota (L.) Royen Fruit Peel: A Phytochemical and Pharmacological Review Karle Pravin P, Dhawale Shashikant C SRP. 2019; 10(1): 11-14 » doi: 0.5530/srp.2019.1.2
  • Pharmacognostic and Phytopharmacological Overview on Bombax ceiba Pankaj Haribhau Chaudhary, Mukund Ganeshrao Tawar SRP. 2019; 10(1): 20-25 » doi: 10.5530/srp.2019.1.4
  • A Review of Pharmacoeconomics: the key to “Healthcare for All” Hasamnis AA, Patil SS, Shaik Imam, Narendiran K SRP. 2019; 10(1): s40-s42 » doi: 10.5530/srp.2019.1s.21
  • A Prospective Review on Phyto-Pharmacological Aspects of Andrographis paniculata Govindraj Akilandeswari, Arumugam Vijaya Anand, Palanisamy Sampathkumar, Puthamohan Vinayaga Moorthi, Basavaraju Preethi SRP. 2019; 10(1): 15-19 » doi: 10.5530/srp.2019.1.3