Abstract

Gulboy Abdolmajeed Nasir, Mohammed Ayyed Najm, Ammar Lateef Hussein

The recent studies suggested the possible toxicities or genetic alterations associated with biological and medical applications of silver nanoparticles (AgNPs). The current research is directed to see if AgNPs administration can lead to some changes in expression of BRAF gene in selected body organs tissues. Fifty-six male of musmusculs (Balb/C) mice from the animal house of Al-Nahrain Centre of Biotechnology were used. These animals were divided randomly to seven groups (eight mouse in each group), one of these groups represented the control group, three groups were subjected to different doses of AgNPs (0.25, 0.5and 1 mg/kg of body weight) for one week, and the remaining three groups were subjected to three different doses of AgNPs (0.25, 0.5and 1 mg/kg of body weight) for two weeks. Liver, spleen, brain and kidneys tissue samples from each mouse in all groups (including control group) were collected, RNA was extracted and cDNAs were synthesized and then used for real time PCR and qRT-PCR Analysis. The data generated in this study indicates that the hepatic and spleen tissues expression of BRAF gene is significantly linked to AgNPs administration, also, these data showed a high significant relation between AgNPs administration and the expression of this gene in brain and kidneys tissues.

How to Cite this Article

Pubmed Style Nasir GA, Najm MA, Hussein AL. The Effect of Silver Nanoparticles on BRAF Gene Expression. SRP. 2020; 11(10): 570-575. doi:10.31838/srp.2020.10.85 Web Style Nasir GA, Najm MA, Hussein AL. The Effect of Silver Nanoparticles on BRAF Gene Expression. http://www.sysrevpharm.org/?mno=2772 [Access: March 30, 2021]. doi:10.31838/srp.2020.10.85 AMA (American Medical Association) Style Nasir GA, Najm MA, Hussein AL. The Effect of Silver Nanoparticles on BRAF Gene Expression. SRP. 2020; 11(10): 570-575. doi:10.31838/srp.2020.10.85 Vancouver/ICMJE Style Nasir GA, Najm MA, Hussein AL. The Effect of Silver Nanoparticles on BRAF Gene Expression. SRP. (2020), [cited March 30, 2021]; 11(10): 570-575. doi:10.31838/srp.2020.10.85 Harvard Style Nasir, G. A., Najm, . M. A. & Hussein, . A. L. (2020) The Effect of Silver Nanoparticles on BRAF Gene Expression. SRP, 11 (10), 570-575. doi:10.31838/srp.2020.10.85 Turabian Style Nasir, Gulboy Abdolmajeed, Mohammed Ayyed Najm, and Ammar Lateef Hussein. 2020. The Effect of Silver Nanoparticles on BRAF Gene Expression. Systematic Reviews in Pharmacy, 11 (10), 570-575. doi:10.31838/srp.2020.10.85 Chicago Style Nasir, Gulboy Abdolmajeed, Mohammed Ayyed Najm, and Ammar Lateef Hussein. "The Effect of Silver Nanoparticles on BRAF Gene Expression." Systematic Reviews in Pharmacy 11 (2020), 570-575. doi:10.31838/srp.2020.10.85 MLA (The Modern Language Association) Style Nasir, Gulboy Abdolmajeed, Mohammed Ayyed Najm, and Ammar Lateef Hussein. "The Effect of Silver Nanoparticles on BRAF Gene Expression." Systematic Reviews in Pharmacy 11.10 (2020), 570-575. Print. doi:10.31838/srp.2020.10.85 APA (American Psychological Association) Style Nasir, G. A., Najm, . M. A. & Hussein, . A. L. (2020) The Effect of Silver Nanoparticles on BRAF Gene Expression. Systematic Reviews in Pharmacy, 11 (10), 570-575. doi:10.31838/srp.2020.10.85