Abstract

Ali M Abdelaal, Suhad S Mahmood.

The rapid development of Acinetobacter baumannii resistant to almost all antibiotics even Colistin was making these bacteria a significant public health problem. This study aimed to determine the role of adeJ gene of RND-efflux pump, and it is a correlation with levofloxacin resistance in Acinetobacter baumannii. A total of 130 bacterial isolates were collected from patients admitted to Baghdad hospitals. Isolates were identified biochemically and conformed with Vitec 2 compact system, and only 70 isolates were confirmed as Acinetobacter baumannii. The Disc diffusion method for 8 types of antibiotics was used in this study to determine the antibiotics susceptibility for all isolate of A.baumannii. The results of antibiotics susceptibility test showed that the bacterium was resistance to Amikacin in percentage (61.42%). While it is resistant to Trimethoprim was (88.57%) followed by Piperacillin, Cefepime (82.85%), (71.42%) respectively. On the other hand, the resistance to Tetracycline, Ampicillin-sulbactam, Imipenem and levofloxacin was (47.14%), (61.42%), (54.28%), (51.42%) respectively. In addition, only 15 isolates were selected to determine the minimum inhibitory concentration of levofloxacin, and these 15 isolates were chosen because they were resistant to most of the antibiotics tested and it is MIC to Levofloxacin was ranging between 64 µg/100 µl to 128 µg/100 µl. Real-time PCR was used to determine the relative expression of adeJ gene of RND-adeIJK and its correlation with levofloxacin resistance in A.baumannii. The result shows the relative mean of adeJ was 3.1 for resistance group before exposing to levofloxacin and was 7.20 for resistance A.baumannii after exposing to levofloxacin and show significant difference (P=0.039). This study indicates that AdeJ has a role in providing resistance to levofloxacin .In addition; the overexpression of adeJ may be one of the reasons contributing to Resistance to a wide variety of antibiotics.

How to Cite this Article

Pubmed Style Abdelaal AM, Mahmood SS. The Role of Efflux Pump adeJ Gene in Levofloxacin Resistance among A.baumannii. SRP. 2020; 11(10): 1105-1110. doi:10.31838/srp.2020.10.159 Web Style Abdelaal AM, Mahmood SS. The Role of Efflux Pump adeJ Gene in Levofloxacin Resistance among A.baumannii. http://www.sysrevpharm.org/?mno=55245 [Access: March 29, 2021]. doi:10.31838/srp.2020.10.159 AMA (American Medical Association) Style Abdelaal AM, Mahmood SS. The Role of Efflux Pump adeJ Gene in Levofloxacin Resistance among A.baumannii. SRP. 2020; 11(10): 1105-1110. doi:10.31838/srp.2020.10.159 Vancouver/ICMJE Style Abdelaal AM, Mahmood SS. The Role of Efflux Pump adeJ Gene in Levofloxacin Resistance among A.baumannii. SRP. (2020), [cited March 29, 2021]; 11(10): 1105-1110. doi:10.31838/srp.2020.10.159 Harvard Style Abdelaal, A. M. & Mahmood, . S. S. (2020) The Role of Efflux Pump adeJ Gene in Levofloxacin Resistance among A.baumannii. SRP, 11 (10), 1105-1110. doi:10.31838/srp.2020.10.159 Turabian Style Abdelaal, Ali M, and Suhad S Mahmood. 2020. The Role of Efflux Pump adeJ Gene in Levofloxacin Resistance among A.baumannii. Systematic Reviews in Pharmacy, 11 (10), 1105-1110. doi:10.31838/srp.2020.10.159 Chicago Style Abdelaal, Ali M, and Suhad S Mahmood. "The Role of Efflux Pump adeJ Gene in Levofloxacin Resistance among A.baumannii." Systematic Reviews in Pharmacy 11 (2020), 1105-1110. doi:10.31838/srp.2020.10.159 MLA (The Modern Language Association) Style Abdelaal, Ali M, and Suhad S Mahmood. "The Role of Efflux Pump adeJ Gene in Levofloxacin Resistance among A.baumannii." Systematic Reviews in Pharmacy 11.10 (2020), 1105-1110. Print. doi:10.31838/srp.2020.10.159 APA (American Psychological Association) Style Abdelaal, A. M. & Mahmood, . S. S. (2020) The Role of Efflux Pump adeJ Gene in Levofloxacin Resistance among A.baumannii. Systematic Reviews in Pharmacy, 11 (10), 1105-1110. doi:10.31838/srp.2020.10.159