Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum
Abstract
Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed
A simple ZIC-HILIC-UV technique was developed and applied for the determination of 2-deoxyguanosine in the human serum. The separa-tion took place in the ZIC1 and ZIC5 self-designed stationary phases, in gradient elution mode with a buffer for acetonitrile acetate and 254 nm UV detection. The 2-deoxyguanosine conduct with varying acetate buffer, ACN and pH values has been tested and the results have established the hydrophilicity of 2-deoxyguanosine. The mechanism of separation is based on the hydrophilic and ion exchange partitioning of the2-deoxyguanosine. We have defined and mentioned the current effect of both ZIC1 and ZIC5 stationary stationary phenomena on chromatographic conditions (sodium acetate buffer concentration, ACN and pH). All existing methods are a useful alternative to the current 2-deoxyguanosine separation methods.
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| Pubmed Style Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed. Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum. SRP. 2020; 11(3): 240-246. doi:10.5530/srp.2020.3.27 Web Style Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed. Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum. http://www.sysrevpharm.org/?mno=92890 [Access: March 29, 2021]. doi:10.5530/srp.2020.3.27 AMA (American Medical Association) Style Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed. Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum. SRP. 2020; 11(3): 240-246. doi:10.5530/srp.2020.3.27 Vancouver/ICMJE Style Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed. Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum. SRP. (2020), [cited March 29, 2021]; 11(3): 240-246. doi:10.5530/srp.2020.3.27 Harvard Style Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed (2020) Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum. SRP, 11 (3), 240-246. doi:10.5530/srp.2020.3.27 Turabian Style Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed. 2020. Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum. Systematic Reviews in Pharmacy, 11 (3), 240-246. doi:10.5530/srp.2020.3.27 Chicago Style Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed. "Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum." Systematic Reviews in Pharmacy 11 (2020), 240-246. doi:10.5530/srp.2020.3.27 MLA (The Modern Language Association) Style Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed. "Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum." Systematic Reviews in Pharmacy 11.3 (2020), 240-246. Print. doi:10.5530/srp.2020.3.27 APA (American Psychological Association) Style Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed (2020) Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum. Systematic Reviews in Pharmacy, 11 (3), 240-246. doi:10.5530/srp.2020.3.27 |
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