Andrea Fera

Department of Biological Sciences, Geri Anderson and Associates Inc, Fulton, United States of America

Publications

  • Research Article   
    High-Resolution Electron Microscopy Tomography of Interacting Flash-Frozen Proteins
    Author(s): Andrea Fera37007*

    Electron microscopy tomography results obtained after increasing the resistance of flash-frozen proteins to accelerated electron beams. This curing reduces electron dose dependence, allowing high-resolution tomography without need of averaging multiple samples. Three-dimensional images at atomic-scale are realized on one individual protein in one experiment. This workflow, introduced for flash-frozen rigid biopolymers, is here generalized and applied to flash-frozen, deformable oligomers extracted from a virus. Results are shown down to atomic coordinates without recurring to averaging among many samples. It is possible, therefore, to measure unique, individual enzymes during interactions with proteins. In fact, results like this are usual when imaging by electron microscopy semiconductors or other solid materials, because such samples are not.. Read More»
    DOI: 10.31858/0975-8453.13.1.48-51

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