Indra Sen Singh
Department of Chemistry, School of Mathematics and Natural Sciences, The Copperbelt University, Kitwe, ZambiaPublications
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Research Article
Phytochemical and LCMS/MS Screening, Total Phenolic and Flavonoid Content and Antioxidant Activity of the Leaves of Diospyros batokana (Ebenaceae)
Author(s): Bitwell Chibuye53576*, Indra Sen Singh53578, Luke Chimuka53583 and Kakoma Maseka53584
Diospyros batokana (Ebenaceae) is used traditionally in Zambia to treat various ailments among degenerative diseases. This study aimed to screen the phytochemicals using chemical tests and LCMS/MS and evaluate the total phenolic and flavonoid contents and antioxidant activity of methanolic leaf extract of Diospyros batokana. Whereas maceration was used to extract phytocompounds for preliminary phytochemical screening, QuEChERS based method was used to extract polyphenolics in methanol for LCMS/MS screening. Total phenolic content was evaluated using the Folin-Ciocalteu method, at 765 nm. Total flavonoid content was estimated by aluminium chloride colorimetric assay at 510 nm. Antioxidant activity was determined using 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) free radical scavenging method, at 517 nm. Preliminary phytochemi.. Read More»
DOI: 10.31858/0975-8453.14.2.105-112
Abstract
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