Sejalben Patel
Department of Quality Assurance and Pharma Chemistry, Nootan Pharmacy College, Sankalchand Patel University, Gujarat, IndiaPublications
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Research Article
Development of Validated Stability-Indicating Chromatographic Method for the Determination of Nebivolol and Valsartan and its Related Impurities in Pharmaceutical Tablets
Author(s): Vaishali Gohel59990*, Sejalben Patel59991 and Ujashkumar Shah59992
A simple, economical, precise, and selective Reverse gradient Phase High-Performance Liquid Chromatography (RP-HPLC) method has been validated and developed to estimate related impurities of Nebivolol (NEB) and Valsartan (VAL) in a tablet dosage form. A RP-HPLC analysis was performed on Hypersil BDS C18 column, and its size was 250 mm × 4.6 mm, 5 μm with using mobile phase Acetonitrile and Potassium dihydrogen phosphate (KH2PO4) with buffer pH-3.0 in the ratio of (50:50) at 282 nm detection wavelength with the flow rate of 1.0 mL/min. The analytical method was validated according to International Council for Harmonisation (ICH) guidelines. The linearity was observed in the Limit of 25-75 μg/ml range for Nebivolol and 1-15 μg/ml range for its related impurity A and B. Similarly, the 400-1200 μg/mL range w.. Read More»
DOI: 10.31858/0975-8453.14.6.411-417
Abstract
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